Effects of Different Probiotics on Intestinal Microbial Community Structure of Broilers

LI Ke; LUO Jianjie; MENG Kun; YAO Bin; LIU Guohua; ZHENG Aijuan

doi:10.3969/j.issn.1006-267x.2015.11.024

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 11: 3516 - 3526

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.11.024

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Effects of Different Probiotics on Intestinal Microbial Community Structure of Broilers

LI Ke ,
LUO Jianjie ,
MENG Kun ,
YAO Bin ,
LIU Guohua ,
ZHENG Aijuan

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1. Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. Beijing Creat-Value Biological Technology Co., Ltd., Beijing 100086, China

Received date: 2015-05-06

Online published: 2015-11-21

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Abstract

This experiment was conducted to study the effects of different probiotics(Bacillus licheniformis, Enterococcus faecium and Clostridium butyricum) on microbial community structure of broilers. A total of 432 one-day-old roosters were randomly allocated to 4 groups, group 1 was fed the basal diet, groups 2 to 4 were fed the basal diet with Bacillus licheniformis (≥4×10⁶ CFU/g), Enterococcus faecium (≥10⁶ CFU/g) and Clostridium butyricum (≥10⁶ CFU/g), respectively. Diversity of intestinal microflora was determined based on V3 variable regions of bacteria 16S rDNA by using PCR-denaturing gradient gel electrophoresis (DGGE) technique. The flora amount of bacteria in the intestine was quantified by real-time quantitative PCR (qPCR). The PCR-DGGE fingerprints results showed as follows:compared with the control group, Enterococcus could significantly increase the number of bands in PCR-DGGE fingerprints (P<0.05), while the number of bands in PCR-DGGE fingerprints of other group was not significantly affected (P<0.05). From the brightness of bands in PCR-DGGE fingerprints, we could know that:Enterococcus faecium was successfully colonized in the ileum, and promote the growth of Lactobacillus, Bacillus licheniformis, also promote the growth of Lactobacillus with a synergistic effect, and inhibit the growth of C. eutactus and C. irregulare with a competition effect. Clostridium butyricum promote the growth of L. aviaries in jejunum and L. versmoldensis in ileum with a more specific effect. The qPCR results showed as follows:the flora amount of bacteria in jejunum, ileum and cecum of Bacillus licheniformis group was 1.5, 1.0 and 1.3 times as many as that of control group; the flora amount of bacteria in jejunum, ileum and cecum of Enterococcus faecium group was 4.0, 6.0 and 0.6 times as many as that of control group; the flora amount of bacteria in jejunum, ileum and cecum of Clostridium butyricum group was 1.0, 3.5 and 0.5 times as many as that of control group. In conclusion, Bacillus licheniformis, Enterococcus faecium and Clostridium butyricum can change the micro-ecology environment of broiler intestine to varying degrees.

Key words： probiotics; denaturing gradient gel electrophoresis; real-time quantitative PCR; intestine; microbial community structure

Cite this article

LI Ke , LUO Jianjie , MENG Kun , YAO Bin , LIU Guohua , ZHENG Aijuan . Effects of Different Probiotics on Intestinal Microbial Community Structure of Broilers[J]. Chinese Journal of Animal Nutrition, 2015 , 27(11) : 3516 -3526 . DOI: 10.3969/j.issn.1006-267x.2015.11.024

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