This study was conducted to investigate the effects of Honeysuckle extract on antioxidant function and skeletal muscle morphology of beef cattle under hot environment. Ten healthy Jinjiang yellow cattle with[(360±20) kg] were randomly divided into 2 groups with 5 heads per each group. Animals were fed under environment of high temperature and humidity in summer. Honeysuckle extract (0.2%) was added in concentrate for cattle in Honeysuckle group. The test lasted for 60 days. The results showed as follows:1) high temperature and humidity environment in summer caused heat stress of beef cattle, the average temperature humidity index (THI) was above 79. 2) No difference was noticed about average daily gain of beef cattle between two groups (P>0.05). 3) Compared with control group, serum glutathione peroxidase (GSH-Px) activity and total antioxidant capacity (T-AOC) in Honeysuckle group were significantly increased (P<0.05), malondialdehyde (MDA) content was significantly decreased (P<0.05), creatine kinase (CK) activity was decreased by 105.09% (P<0.05), and the activities of total superoxide dismutase (T-SOD) and lactate dehydrogenase (LDH) were not affected (P>0.05). 4) Compared with control group, T-AOC of skeletal muscle in Honeysuckle group was increased by 59.74% (P<0.05), and sarcomere length was increased by 13.16% (P<0.05); skeletal muscle morphosis in control group was damaged, and appeared swelling and disfiguration, while the damage was restored in Honeysuckle group. These results indicate that high temperature and humidity environment induced heat stress reaction of beef cattle, however, dietary supplementation of Honeysuckle extract improved the antioxidant and restored the morphosis of damaged muscle.