Diversity of Bacterial Communities in Rumen Fluid of Dairy Cows Affected by Forage Sources in Diet

XU Jun; HU Lifang; HOU Yujie; SUN Jianyong; ZHAO Guoqi

doi:10.3969/j.issn.1006-267x.2015.11.028

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 11: 3549 - 3557

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.11.028

SHORT COMMUNICATIONS

Diversity of Bacterial Communities in Rumen Fluid of Dairy Cows Affected by Forage Sources in Diet

XU Jun ,
HU Lifang ,
HOU Yujie ,
SUN Jianyong ,
ZHAO Guoqi

Expand

1. Institute of Quality Safety and Standards of Agricultural Products, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China;
2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
3. Institute of Animal Husbandry and Vet of State-Owned First Pasture of Xinjiang, Aletai 836500, China

Received date: 2015-06-01

Online published: 2015-11-21

Fold

Abstract

This study was conducted to investigate the effects of forage sources in diet on the diversity of bacterial communities in rumen fluid of dairy cows. Eight Holstein dairy cows [(632±12) kg of body weight; (135±16) days in milk] were used in a replicated 4×4 Latin square design, and each group contained two cows. Oat hay, Leymus chinensis, rice straw and alfalfa were used as forage sources to form four isonitrogenous and isocaloric diets, which were based on corn silage and had equivalent neutral detergernt fiber (NDF) and non-fiber carbohydrates (NFC) contents; each test period lasted for 21 days with the first 14 days for adaption. The results showed as follows:1) Bacteroidertes, Firmicutes, Spirochaetes and Fibrobacteres were the most abundant in rumen under phyla level; Prevotella, Butyrivibrio, Fibrobacter and Treponema were the most abundant under genus level. 2) Similarity of the bacterial community feeding the same diet was high under genus level, and the genetic distance was near in rumen fluid of cows fed oat hay, rice straw and alfalfa. 3) The four groups comprised of 9 329 operational taxonomic units (OTUs) at the 97% similarity level and 4 120 OTUs were shared, which accounted for 44.16% of the total OTUs. The results indicate that cows fed diets with different forage sources with equivalent NDF and NFC contents have similar diversity of bacterial communities.

Key words： bacterial community; high throughput sequencing; forage sources; rumen

Cite this article

XU Jun , HU Lifang , HOU Yujie , SUN Jianyong , ZHAO Guoqi . Diversity of Bacterial Communities in Rumen Fluid of Dairy Cows Affected by Forage Sources in Diet[J]. Chinese Journal of Animal Nutrition, 2015 , 27(11) : 3549 -3557 . DOI: 10.3969/j.issn.1006-267x.2015.11.028

References

[1] SUN X Z, HOSKIN S O, JOBLIN K N, et al.Forage cell walls:ruminal degradation[J].Acta Agrestia Sinica, 2007, 15(4):386-393.
[2] PITTA D W, PINCHAK W E, DOWD S E, et al.Rumen bacterial diversity dynamics associated with changing from bermudagrass hay to grazed winter wheat diets[J].Microbial Ecology, 2010, 59(3):511-522.

[3] BELANCHE A, DOREAU M, EDWARDS J E, et al.Shifts in the rumen microbiota due to the type of carbohydrate and level of protein ingested by dairy cattle are associated with changes in rumen fermentation[J].The Journal of Nutrition, 2012, 142(9):1684-1692.

[4] JONES R T, ROBESON M S, LAUBER C L, et al.A comprehensive survey of soil acidobacterial diversity using pyrosequencing and clone library analyses[J].The ISME Journal, 2009, 3(4):442-453.

[5] THOETKIATTIKUL H, MHUANTONG W, LAOTHANACHAREON T, et al.Comparative analysis of microbial profiles in cow rumen fed with different dietary fiber by tagged 16S rRNA gene pyrosequencing[J].Current Microbiology, 2013, 67(2):130-137.

[6] TAJIMA K, ARAI S, OGATA K, et al.Rumen bacterial community transition during adaptation to high-grain diet[J].Anaerobe, 2000, 6(5):273-284.

[7] MOSONI P, FONTY G, GOUET P.Competition between ruminal cellulolytic bacteria for adhesion to cellulose[J].Current Microbiology, 1997, 35(1):44-47.

[8] JAMI E, MIZRAHI I.Composition and similarity of bovine rumen microbiota across individual animals[J].PLoS One, 2012, 7(3):e33306.
[9] DE MENEZES A B, LEWIS E, O'DONOVAN M, et al.Microbiome analysis of dairy cows fed pasture or total mixed ration diets[J].FEMS Microbiology Ecology, 2011, 78(2):256-265.

[10] MEYER M, STENZEL U, HOFREITER M.Parallel tagged sequencing on the 454 platform[J].Nature Protocols, 2008, 3(2):267-278.

[11] MATSUI H, OGATA K, TAJIMA K, et al.Phenotypic characterization of polysaccharidases produced by four Prevotella type strains[J].Current Microbiology, 2000, 41(1):45-49.

[12] KAMRA D N.Rumen microbial ecosystem[J].Current Science, 2005, 89(1):124-135.
[13] TAJIMA K, AMINOV R I, NAGAMINE T, et al.Rumen bacterial diversity as determined by sequence analysis of 16S rDNA libraries[J].FEMS Microbiology Ecology, 1999, 29(2):159-169.

[14] EVANS N J, BROWN J M, MURRAY R D, et al.Characterization of novel bovine gastrointestinal tract Treponema isolates and comparison with bovine digital dermatitis treponemes[J].Applied and Environmental Microbiology, 2011, 77(1):138-147.

[15] KOPECNY J, ZOREC M, MRÁZEK J, et al.Butyrivibrio hungatei sp.nov.and Pseudobutyrivibrio xylanivorans sp.nov., butyrate-producing bacteria from the rumen[J].International Journal of Systematic and Evolutionary Microbiology, 2003, 53(1):201-209.

[16] CUNHA I S, BARRETO C C, COSTA O Y A, et al.Bacteria and Archaea community structure in the rumen microbiome of goats (Capra hircus) from the semiarid region of Brazil[J].Anaerobe, 2011, 17(3):118-124.

[17] STEVENSON D M, WEIMER P J.Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR[J].Applied Microbiology and Biotechnology, 2007, 75(1):165-174.

[18] KOCHERGINSKAYA S A, AMINOV R I, WHITE B A.Analysis of the rumen bacterial diversity under two different diet conditions using denaturing gradient gel electrophoresis, random sequencing, and statistical ecology approaches[J].Anaerobe, 2001, 7(3):119-134.

[19] WHITFORD M F, FORSTER R J, BEARD C E, et al.Phylogenetic analysis of rumen bacteria by comparative sequence analysis of cloned 16S rRNA Genes[J].Anaerobe, 1998, 4(3):153-163.

[20] HESS M, SCZYRBA A, EGAN R, et al.Metagenomic discovery of biomass-degrading genes and genomes from cow rumen[J].Science, 2011, 331(6016):463-467.

[21] XU J, HOU Y J, YANG H B, et al.effects of forage sources on rumen fermentation characteristics, performance, and microbial protein synthesis in midlactation cows[J].Asian-australasian Journal of Animal Sciences, 2014, 27(5):667-673.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References