Inhibiting Effects of Condensed Tannins from Purple Prairie Clover on Escherichia coli

LIU Xiuli; WANG Yuxi; LI Yuanheng; XU Zhongjun; JIN Long; HOU Yongyue

doi:10.3969/j.issn.1006-267x.2015.11.036

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 11: 3620 - 3627

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.11.036

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Inhibiting Effects of Condensed Tannins from Purple Prairie Clover on Escherichia coli

LIU Xiuli ,
WANG Yuxi ,
LI Yuanheng ,
XU Zhongjun ,
JIN Long ,
HOU Yongyue

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1. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China;
2. Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge T1J 4B1, Canada;
3. Institute of Grassland Research of CAAS, Hohhot 010010, China;
4. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2015-05-27

Online published: 2015-11-21

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Abstract

This experiment was conducted to evaluate inhibiting effects of purple prairie clover (PPC, Dalea purpurea Vent.) on Escherichia coli (E. coli) growth prevalence in the rumen and large intestine and its condensed tannins (CT) antibacterial mechanism. Ruminal and large intestinal environments were simulated in vitro, non-pathogenic E. coli ATCC 25922 and pathogenic E. coli O157:H7 were used as test strains, and the strains were cultured with PPC and alfalfa (no CT, control) as substrates, respectively. Bacteria number was determined after cultured for 0, 1.5, 3.0, 6.0 and 24.0 h, respectively. With the E. coli ATCC 25922 as test model, the bacteria suspensions supplemented with CT from PPC and sainfoin were mixed with o-nitrophenyl-β-D-galactoside (ONPG) solution, meanwhile, the mixture of bacteria suspension without CT and ddH₂O was used as control. O-nitrophenyl amount, the hydrolysis product of β-D-galactosidase, was determined after cultured for 0, 40, 80, 120 and 160 min, respectively, to analysis the change of cell inner-membrane permeability. The effects of different pH and temperatures on CT actions were also observed. The results revealed as follows:1) compared with alfalfa, PPC had significant inhibiting effect on growth of E. coli from rumen (6.0 and 24.0 h) and large intestine (1.5 to 24.0 h) (P<0.05). 2) CT significantly increased o-nitrophenyl amount (P<0.05); differently from actions of CT from sainfoin, actions of CT from PPC were affected by pH and temperature. The results indicate that PPC showed inhibiting effect on E. coli growth in bovine rumen and large intestine in vitro incubation; its CT can increase inner membrane permeability of E. coli, however, action of inhibition associated with temperature and pH.

Key words： PPC; condensed tannins; E. coli; inhibition

Cite this article

LIU Xiuli , WANG Yuxi , LI Yuanheng , XU Zhongjun , JIN Long , HOU Yongyue . Inhibiting Effects of Condensed Tannins from Purple Prairie Clover on Escherichia coli[J]. Chinese Journal of Animal Nutrition, 2015 , 27(11) : 3620 -3627 . DOI: 10.3969/j.issn.1006-267x.2015.11.036

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