A Study on the Resistance of Microencapsulated Probiotics by the Emulsion and Internal Gelation Method

ZHANG Lin; YUN Tingting; QI Wentao; LI Jie; WANG Yongwei; LI Aike

doi:10.3969/j.issn.1006-267x.2015.11.038

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 11: 3636 - 3642

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.11.038

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A Study on the Resistance of Microencapsulated Probiotics by the Emulsion and Internal Gelation Method

ZHANG Lin ,
YUN Tingting ,
QI Wentao ,
LI Jie ,
WANG Yongwei ,
LI Aike

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1. Animal Nutrition and Feed Science, Northeast Agricultural University, Harbin, 150030, China;
2. Academy of State Administration of Grain, Beijing 100037, China

Received date: 2015-05-17

Online published: 2015-11-21

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Abstract

The Saccharomyces boulardii and Enterococcus faecium were microencapsulated by the method of emulsion and internal gelation, and the resistance of the microencapsulated bacteria to the storage, high temperature, simulated gastric and intestinal conditions were evaluated in this paper. Probiotic products were treated at 110 or 130℃ for 30, 45 and 60 s, respectively; and were treated in the simulated gastric or intestinal fluid for 30, 90 and 180 min, respectively. Bacteria powder without microencapsulation was used as control. The results showed as follows:1) the survival rates of microencapsulated Saccharomyces boulardii and Enterococcus faecium were 34.63% and 19.46% higher than those of bacteria powder in five months storage, respectively. 2) The resistance of probiotics to the high temperature at 110 and 130℃ was increased by the microencapsulation, especially that of Enterococcus faecium was significantly increased than bacteria powder (P<0.05). 3) There were 59.18% and 51.80% increasing in the survived rates of microencapsulated Saccharomyces boulardii and Enterococcus faecium compared with those of bacteria powder when the probiotics were treated in the simulated gastric conditions for 30 min, respectively, and the datas were 57.76% and 46.73% when treated for 180 min, respectively. 4) There were 26.89% and 21.16% increasing in survived rate of microencapsulated Saccharomyces boulardii and Enterococcus faecium compared with those of bacteria powder when the probiotics were treated in the simulated intestinal conditions for 180 min, respectively. 5) The release rate of microencapsulated Saccharomyces boulardii and Enterococcus faecium reached 71.74% and 87.47% after 50 min, respectively. These results suggest that the microcapsulation prepared by the emulsion and internal gelation can be an effective way for protecting probiotic bacteria, and can increase the resistance to the storage, high temperature, simulated gastric and intestinal conditions, meanwhile, slow-release in simulated gastrointestinal conditions.

Key words： emulsification gelation; microencapsulated probiotic; stress resistance; Saccharomyces boulardii; Enterococcus faecium

Cite this article

ZHANG Lin , YUN Tingting , QI Wentao , LI Jie , WANG Yongwei , LI Aike . A Study on the Resistance of Microencapsulated Probiotics by the Emulsion and Internal Gelation Method[J]. Chinese Journal of Animal Nutrition, 2015 , 27(11) : 3636 -3642 . DOI: 10.3969/j.issn.1006-267x.2015.11.038

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