Effects of Dietary Crude Protein Level on Gastrointestinal Secretion and Serum Hormone levels of Weaning Piglets

CUI Zhijie; WANG Lijian; WU Fei; JIANG Qian; XIE Dongmei; HE Liuqin; LI Yuying; ZHOU Hao; LI Tiejun

doi:10.3969/j.issn.1006-267x.2015.12.007

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 12: 3689 - 3698

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.12.007

SWINE NUTRITION

Effects of Dietary Crude Protein Level on Gastrointestinal Secretion and Serum Hormone levels of Weaning Piglets

CUI Zhijie ,
WANG Lijian ,
WU Fei ,
JIANG Qian ,
XIE Dongmei ,
HE Liuqin ,
LI Yuying ,
ZHOU Hao ,
LI Tiejun

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1. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Engineering Research Center of Healthy Livestock, Scientifc Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China;
2. University of Chinese Academy of Sciences, Bejing 100039, China;
3. Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625047, China;
4. Hunan Diyi Biotechnology Co., Ltd., Changsha 410148, China

Received date: 2015-06-19

Online published: 2015-12-14

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Abstract

The study was conducted to evaluate the effects of different dietary crude protein levels on gastrointestinal internal environment change, gastrointestinal hormone levels of weaning piglets. Twenty-eight weaning piglets(Duroc×Large White×Landrace) with an initial body weight of(10.0±1.0) kg were randomly divided into four groups. Four diets containing 0(NFP), 14%(LP), 17%(MP), and 20%(HP) crude protein were fed, 7 replicates per treatment, 1 pig per replicate. Each piglet was placemented gastric fistula and carotid artery through surgical operation. And each pig was taken care for 5 d after surgery. Collecting arterial serum according to the time point before the meal(0 min), and 30, 60, 120, 180, and 300 min after fed from 6th to 8th days. And gastric chime was collected according to the same time point with serum from 13th to 17th days. Every pig was collected 1 time point every day except 0 and 300 min. Serum hormone levels, liquid supernatant of gastric chime, and pepsin activity were determined. All piglets were limited to feed, 450 g per day and were free to water except the time when collecting gastric chime. And the ratio of feed to water is 2 to 1 when collecting gastric chime. The results showed that the average daily gain(ADG) had no significant difference among HP, MP, and LP groups(P>0.05), piglets fed with NFP diet had a negative growth in ADG, and had significant difference with the other groups(P<0.05) in the case of restricted feeding. With the decrease of crude protein level, the length of small intestine was declined(P>0.05) and the gastrointestinal evacuation rate was accelerated(P>0.05). Serum levels of cholecystokinin(CCK), ghrelin, gastrin, gastric inhibitory polypeptide(GIP), pepsinogen Ⅰ(PG Ⅰ), and pepsinogen Ⅱ(PG Ⅱ) were not or little influenced by crude protein level and intake time(P>0.05). These results indicate that the 6% decrease of dietary crude protein level do not influence the pH, pepsin activity and serum hormone levels of weaning piglets compared with common diet(crude protein is 20%) in the case of restricted feeding.

Key words： crude protein level; weaning piglets; gastric acid; serum hormones

Cite this article

CUI Zhijie , WANG Lijian , WU Fei , JIANG Qian , XIE Dongmei , HE Liuqin , LI Yuying , ZHOU Hao , LI Tiejun . Effects of Dietary Crude Protein Level on Gastrointestinal Secretion and Serum Hormone levels of Weaning Piglets[J]. Chinese Journal of Animal Nutrition, 2015 , 27(12) : 3689 -3698 . DOI: 10.3969/j.issn.1006-267x.2015.12.007

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