This experiment was conducted to study the effects of different supplementation of dietary Tenebrio molitor L. on growth performance, nutrient digestibility and serum biochemical parameters of minks at the earlier development period. The trial was taken the single factor method, sixty 60-day-old minks were randomly assigned into 5 groups with 12 replicates per group and 1 replicate per group. H0 group fed with the basic diet without Tenebrio molitor L., H4, H8, H12, H16 groups fed diets supplemented with 4%, 8%, 12% and 16% Tenebrio molitor L., respectively. The pre-test period lasted for 7 days and the trial period lasted for 60 days. The results showed as follows:1) from 2013-08-05 to 2013-09-15, the body weight of H0 group were significantly higher than that of H16 group(P<0.05), the other groups was no significant difference on body weight of minks(P>0.05). 2) The digestibility of dry matter, protein and fat were no significant difference among all groups(P>0.05). 3) The nitrogen intake and fecal nitrogen output were no significant difference among all groups(P>0.05), the urinary nitrogen excretion of H4 group was significantly lower than that of H12 group(P<0.05). The nitrogen deposition, net protein utilization and biological value of protein of H4 group were significantly higher than that of H12 group(P<0.05). 4) The serum cholesterol content of H4, H8 and H16 groups was significantly higher than that of H0 group(P<0.01). The serum calcium content of H4 and H8 groups was significantly higher than that of H12 group(P<0.05), while the serum phosphorus content of H16 group was significantly higher than that of other groups(P<0.05). In conclusion, minks fed with Tenebrio molitor L. do not significantly improve growth performance at the earlier development period. When the fish meal price is higher than Tenebrio molitor L., we can add 4% to 12% Tenebrio molitor L. substitute for fish meal, bone meat meal and other animal feed in the diet.