FEED SCIENCE AND TECHNOLOGY

Effects of Probiotics on Growth Performance, Digestive Enzyme and Immune Enzyme Activities of Juvenile Cobia(Rachycentron canadum)

  • HE Weicong ,
  • DONG Xiaohui ,
  • TAN Beiping ,
  • YANG Qihui ,
  • CHI Shuyan ,
  • LIU Hongyu ,
  • ZHANG Shuang
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  • 1. Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;
    2. South China Sea Bio-Resource Exploitation and UtilizationCollaborative Innovation Center, Guangzhou 510006, China

Received date: 2015-06-24

  Online published: 2015-12-14

Abstract

This experiment was conducted to study the effects of Bacillus subtilis and Lactobacillus acidophilus as separate or mixed in diets on the growth performance, digestive enzyme and immune enzyme activities of juvenile cobia(Rachycentron canadum). Eight hundred and forty healthy juvenile cobia with an initial body weight of(22.00±0.15) g were randomly divided into 7 groups(groups T0, T1, T2, T3, T4, T5 and T6, respectively) with 3 replicates per group and 40 fish per replicate. The group T0 was used as control group, and fish in this group were fed a basal diet for 50 days; the fish in groups T1, T2 and T3 were fed diets containing 1×107 CFU/g B. subtilis, 1×107 CFU/g L. acidophilus and 2×107 CFU/g B. subtilis and L. acidophilus mixture(mass ratio of B. subtilis and L. acidophilus=1:1) for 30 days and then 20 days switched to basal diet, respectively; the fish in groups T4, T5 and T6 were fed diets containing 1×107 CFU/g B. subtilis, 1×107 CFU/g L. acidophilus and 2×107 CFU/g B. subtilis and L. acidophilus mixture for 50 days, respectively. The results showed as follows:compared with control group, the weight gain rate(WGR) in groups T1 and T2 had no significant difference(P>0.05), but the WGR in groups T3, T4, T5 and T6 was significantly increased(P<0.05), and the group T6 had the maximum value which was significantly higher than that in other groups(P<0.05). The specific gain rate(SGR) had a similar changing trend to WGR. The feed conversion ratio(FCR) in groups T2, T3, T4, T5 and T6 was significantly lower than that in control group(P<0.05), meanwhile, the FCR in groups T4 and T6 was significantly lower than that in other probiotics supplemental groups(P<0.05). Diets with supplementation of probiotics could significantly enhance the activities of amylase and protease in liver(P<0.05), and improve the activity of protease in intestine(P<0.05). The activity of amylase in intestine in control goup, groups T3 and T4 was significantly lower than that in groups T5 and T6, and significantly higher than that in groups T1 and T2(P<0.05). Diet with supplementation of probiotics could significantly improve the activity of serum lysozyme(LZM)(P<0.05). The serum total superoxide dismutase(T-SOD) activity in continuous feeding groups(groups T4, T5 and T6) was significantly higher than that in control group(P<0.05), and the serum T-SOD activity in group T6 was significantly higher than that in groups T4 and T5(P<0.05). However, the serum T-SOD activity in non-continuous feeding groups(groups T1, T2 and T3) was significantly lower than that in control group except group T1(P<0.05). Compared with the control group, the serum alkaline phosphatase(AKP) activity in probiotics supplemental groups was significantly decreased(P<0.05). The serum acid phosphatase(ACP) activity in groups T2 and T3 was significantly higher than that in control group(P<0.05), and group T6 had no significant difference with control group(P>0.05), while that in other groups was significantly lower than that in control group(P<0.05). These results indicate that supplementation of B. subtilis and L. acidophilus as separate or mixed(1:1, mass ratio) in diets can promote the growth, and increase the activities of immune enzymes and digestive enzymes of juvenile cobia. Continuous feeding is better than non-continuous feeding and the mixed probiotics has the best effect.

Cite this article

HE Weicong , DONG Xiaohui , TAN Beiping , YANG Qihui , CHI Shuyan , LIU Hongyu , ZHANG Shuang . Effects of Probiotics on Growth Performance, Digestive Enzyme and Immune Enzyme Activities of Juvenile Cobia(Rachycentron canadum)[J]. Chinese Journal of Animal Nutrition, 2015 , 27(12) : 3821 -3830 . DOI: 10.3969/j.issn.1006-267x.2015.12.022

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