Effects of Protein Source in Milk Replacer on Nutrient Digestion and Rumen Fermentation of Early-Weaner Calves

HUANG Kaiwu; TU Yan; SI Bingwen; XU Guishan; GUO Jiangpeng; YANG Chuntao; GUO Feng; DIAO Qiyu

doi:10.3969/j.issn.1006-267x.2015.12.036

Chinese Journal of Animal Nutrition >

2015 , Vol. 27 >Issue 12: 3940 - 3950

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.12.036

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Effects of Protein Source in Milk Replacer on Nutrient Digestion and Rumen Fermentation of Early-Weaner Calves

HUANG Kaiwu ,
TU Yan ,
SI Bingwen ,
XU Guishan ,
GUO Jiangpeng ,
YANG Chuntao ,
GUO Feng ,
DIAO Qiyu

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1. Beijing Key Laboratory of Dairy Nutrition, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. College of Animal Science, Tarim University, Alear 843300, China;
3. The Animal Husbandry Station of Beijing, Beijing 100029, China

Received date: 2015-07-08

Online published: 2015-12-14

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Abstract

The objective of this study was to investigate the effects of protein source in milk replacer on nutrient digestion and rumen fermentation of early-weaner calves. Fifty(21±5)-day-old Chinese Holstein bull calves with similar body weight were randomly assigned to 5 groups with 10 calves each. The protein source of control group(MP group) was from milk protein. Calves in 4 experimental groups were fed with milk replacer whose protein contains 70% vegetable protein and 30% milk protein of crude protein(CP). Vegetable protein sources were soybean protein concentrate(SP), wheat gluten(WP), peanut protein concentrate(PP) and rice protein isolate(RP), respectively. The trial lasted for 42 d, and rumen fluid was collected at 28, 42 and 56 days of age; digestion trials were performed at 29 to 35 and 57 to 63 days of age, respectively. The results showed as follows:1) there was no significant difference on the mean of dry matter intake(DMI) from 22 to 63 days of age among groups(P>0.05), while DMI of claves at 57 to 63 days of age of SP and RP groups was significantly higher than the other groups(P<0.05). 2) At 29 to 35 days of age, organic matter(OM) digestibility of MP group was significantly higher than that of WP, PP and RP groups(P<0.05); CP digestibility of MP group was significantly higher than that of the other groups(P<0.05), while SP and RP groups were significantly higher than WP and PP groups(P<0.05), and WP group was significantly higher than PP group(P<0.05); ether extract(EE) digestibility of MP, SP and RP groups was significantly higher than that of WP and PP groups(P<0.05); calcium(Ca) digestibility of MP and RP groups was significantly higher than that of WP and PP groups(P<0.05); phosphorus(P) digestibility of MP group was significantly higher than that of SP, WP and PP groups(P<0.05). At 57 to 63 days of age, OM digestibility of MP group was significantly higher than that of WP and PP groups(P<0.05); CP digestibility of MP group was significantly higher than that of WP, PP and RP groups(P<0.05); Ca digestibility of MP group was significantly higher than that of SP and WP groups(P<0.05); EE digestibility of MP group was not significant different from that of the other groups(P>0.05); there was no significantly difference on P digestibility among five groups(P>0.05). 3) There were no significantly differences on rumen fermentation indexes among five groups at 28 and 42 days of age(P>0.05); at 56 days of age, rumen fluid pH of SP and RP groups was significantly lower than that of WP and PP groups(P<0.05). The difference of total volatile fatty acids(TVFA) concentration of MP with the other groups was not significantly(P>0.05), while TVFA concentration of PP group was significantly lower than that of SP and RP groups(P<0.05); propionic acid and butyric acid concentrations of SP and RP groups were significantly higher and lower than those of MP, WP and PP groups(P<0.05), respectively, and isovaleric acid and valeric acid concentrations were significantly lower than those of the other groups(P<0.05). From the above, under nutrient conditions in the present study, the digestibility of vegetable protein is lower than that of milk protein in calves; soybean protein concentrate has higher digestibility in the four sources of vegetable protein, but the difference is lowered with growing; vegetable protein have stronger auxo-action to rumen growth than milk protein.

Key words： calf; vegetable protein; milk replacer; digestion; metabolism; rumen

Cite this article

HUANG Kaiwu , TU Yan , SI Bingwen , XU Guishan , GUO Jiangpeng , YANG Chuntao , GUO Feng , DIAO Qiyu . Effects of Protein Source in Milk Replacer on Nutrient Digestion and Rumen Fermentation of Early-Weaner Calves[J]. Chinese Journal of Animal Nutrition, 2015 , 27(12) : 3940 -3950 . DOI: 10.3969/j.issn.1006-267x.2015.12.036

References

[1] HEINRICHS A J.Raising dairy replacements to meet the needs of the 21st century[J].Journal of Dairy Science,1993,76(10):3179-3187.

[2] 刁其玉.后备牛蛋白质营养需要研究进展[J].饲料工业,2010(增刊):15-19.
[3] LALLES J P, TOULLEC R, Pardal P B, et al.Hydrolyzed soy protein isolate sustains high nutritional performance in veal calves[J].Journal of Dairy Science,1995,78(1):194-204.

[4] TOMKINS T,SOWINSKE J,DRACKLEY J K.Milk replacer research leads to new developments[J].Feedstuffs,1994,66(42):13-23.
[5] TOMKINS T,SOWINSKI J,DRACKLEY J K.New developments in milk replacers for pre-ruminants[C]//Proceeding 55th Minnesota Nutrition Conference and Roche Technical Symposium. Paul, MN:University of Minnesota, St.,1994:71-89.
[6] 任慧波.植物蛋白代替乳蛋白在犊牛代乳料中的应用研究[D].硕士学位论文.哈尔滨:东北农业大学,2004.
[7] 张丽英.饲料分析及饲料质量检测技术[M].2版.北京:中国农业大学出版社,2003.
[8] ORTIGUES-MARTY I,HOCQUETTE J F,BERTRAND G,et al.The incorporation of solubilized wheat proteins in milk replacers for veal calves:effects on growth performance and muscle oxidative capacity[J].Reproduction Nutrition Development,2003,43:57-76.
[9] LABUSSIERE E,DUBOIS S,VAN MILGEN J,et al.Effect of solid feed on energy and protein utilization in milk-fed veal calves[J].Journal of Animal Science,2009,87(3):1106-1119.
[10] HILL T M,BATEMAN H G,ALDRICH J M,et al.Effects of using wheat gluten and rice protein concentrate in dairy calf milk replacers[J].The Professional Animal Scientist,2008,24(5):465-472.
[11] 李辉.蛋白水平与来源对早期断奶犊牛消化代谢及胃肠道结构的影响[D].博士学位论文.北京:中国农业科学院,2008.
[12] TERNOUTH J H,ROY J H B,STOBO I J F,et al.Concurrent studies of the flow of digesta in the duodenum and of exocrine pancreatic secretion in calves.5.The effect of giving milk once and twice daily,and of weaning[J].British Journal of Nutrition,1977,37(2):237-249.

[13] TUKUR H M,LALLÈS J P,PLUMB G W,et al.Investigation of the Relationship between in vitro ELISA measures of immunoreactive soy globulins and in vivo effects of soy products[J].Journal Agricultural and Food Chemistry,1996,44(8):2155-2161.

[14] 孙泽威,秦贵信,张庆华.大豆抗原蛋白对犊牛生长性能、日粮养分消化率和肠道吸收能力的影响[J].中国畜牧杂志,2005,41(11):30-33.
[15] DAWSON D P,MORRILL J L,REDDY P G,et al.Soy protein concentrate and heated soy flours as protein sources in milk replacer for preruminant calves[J].Journal of Dairy Science,1988,71(5):1301-1309.

[16] GUILLOTEAU P,LE HUËROU-LURON L I,CHAYVIALLE J A,et al.Plasma and tissue levels of digestive regulatory peptides during postnatal development and weaning in the calf[J].Reproduction Nutrition Development,1992,32(3):285-296.

[17] MONTAGNE L,TOULLEC R,LALLÈS J P.Intestinal digestion of dietary and endogenous proteins along the small intestine of calves fed soybean or potato[J].Journal of Animal Science,2001,79(10):2719-2730.
[18] MONTAGNE L,CRÉVIEU-GABRIEL I,TOULLEC R,et al.Influence of dietary protein level and source on the course of protein digestion along the small intestine of the veal calf[J].Journal of Dairy Science,2003,86(3):934-943.

[19] 姚录昆,杨开伦,范苏江,等.12-17日龄羔羊对奶粉及处理大豆粉消化性的研究[J].草食家畜,2000(1):30-32.
[20] 高艳霞.代乳粉中添加大豆蛋白对犊牛生长发育的影响[D].博士学位论文.北京:中国农业科学院,2006.
[21] LIENER I E.Implications of antinutritional components in soybean foods[J].Critical Reviews in Food Science and Nutrition,1994,34(1):31-67.

[22] 李影球.犊牛代乳料中用大豆蛋白替代部分乳蛋白的试验研究[D].硕士学位论文.南宁:广西大学,2005.
[23] 黄锡霞,雒秋江,孟庆龄,等.犊牛对乳和处理大豆粉消化性的比较研究[J].新疆农业大学学报,1997,20(2):9-15.
[24] SUÁREZ B J,VAN REENEN C G,GERRITS W J J,et al.Effects of supplementing concentrates differing in carbohydrate composition in veal calf diets:Ⅱ.Rumen development[J].Journal of Dairy Science,2006,89(11):4376-4386.

[25] 刘洁.肉用绵羊饲料代谢能与代谢蛋白质预测模型的研究[D].博士学位论文.北京:中国农业科学院,2012.
[26] BVRGER P J,PEREIRA J C,VALADARES FILHO S,et al.Ruminal fermentation and microbial efficiency in Holstein calves fed diets with different concentrate levels[J].Revista Brasileira de Zootecnia,2000,29(1):215-224.

[27] 云强.蛋白水平及Lys/Met对断奶犊牛生长、消化代谢及瘤胃发育的影响[D].硕士学位论文.北京:中国农业科学院,2010.
[28] SUÁREZ B J,VAN REENEN C G,BELDMAN G,et al.Effects of supplementing concentrates differing in carbohydrate composition in veal calf diets:Ⅰ.animal performance and rumen fermentation characteristics[J].Journal of Dairy Science,2006,89(11):4365-4375.

[29] 冯仰廉.反刍动物营养学[M].北京:科学出版社,2004.
[30] 胡红莲.奶山羊亚急性瘤胃酸中毒营养生理机制的研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2008.
[31] 刘强,黄应祥,王聪,等.异戊酸对西门塔尔牛瘤胃发酵及尿嘌呤衍生物的影响[J].畜牧兽医学报,2007,38(2):155-160.

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