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Effects of Dietary Concentrate to Forage Ratio on Intestinal Peptide Transporter mRNA Expressions of Calves

  • GUO Jing ,
  • YAO Dandan ,
  • YANG Hongbo ,
  • HUO Yongjiu ,
  • ZHAO Guoqi
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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2015-06-17

  Online published: 2015-12-14

Abstract

This experiment was conducted to observe the effects of concentrate to forage ratio in pellet diet on the mRNA expressions of peptide transporters(PepT1, PepT2, PHT1 and PHT2) in intestinal mucosa of calves. Six Holstein bull calves with similar days of age and body weight were divided into 2 treatments with 3 calves per treatment. Calves were fed pellet diets with concentrate to forage ratio of 75:25(Ⅰ) and 65:35(Ⅱ), respectively. The preliminary trial period was 14 d and the experimental period was 56 d. The results showed as follows:1) the expression abundance of PepT1 mRNA in duodenum and jejunum was significantly higher than that in ileum, cecum and colon(P<0.01), and duodenum and jejunum in treatment Ⅰ were significantly higher than those in treatment Ⅱ(P<0.01). 2) The expression abundance of PepT2 mRNA intestine was little compared with that of PepT1 mRNA. The expression abundance of PepT2 mRNA in duodenum and colon in treatment Ⅰ was significantly higher than that in treatment Ⅱ(P<0.01). 3) The expression abundance of PHT1 mRNA in cecum in treatment Ⅰ was significantly lower than that in treatment Ⅱ(P<0.01), and ileum and colon in treatment Ⅰ were significantly higher than those in treatment Ⅱ(P<0.05). 4) The expression abundance of PHT2 mRNA in cecum in treatment Ⅰ was significantly lower than that in treatment Ⅱ(P<0.01), and duodenum and colon in treatment Ⅰ were significantly higher than those in treatment Ⅱ(P<0.01). The results show that compared with 65:35, concentrate to forage ratio of 75:25 can promote the expressions of PepT1, PepT2, PHT1 and PHT2 mRNA.

Cite this article

GUO Jing , YAO Dandan , YANG Hongbo , HUO Yongjiu , ZHAO Guoqi . Effects of Dietary Concentrate to Forage Ratio on Intestinal Peptide Transporter mRNA Expressions of Calves[J]. Chinese Journal of Animal Nutrition, 2015 , 27(12) : 3951 -3958 . DOI: 10.3969/j.issn.1006-267x.2015.12.037

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