Molecular Nutrition

Effects of Chromium Supplemented Level in Nitrogen-Free Diets on Organ Indexes, Intestinal Mucosa Morphology and Serum Parameters of Rats

  • ZHENG Cancai ,
  • HUANG Yanling
Expand
  • College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China

Received date: 2015-07-23

  Online published: 2016-01-22

Abstract

This experiment was conducted to investigate the effects of different chromium propionate (CrPro) level in nitrogen-free diets (NFD) on organ indexes, intestinal mucosa morphology and serum parameters of rats. According to body weight, a total of 36 weaned Sprague Dawley (SD) rats were allotted to 3 treatments with 6 replicates per treatment and 2 rats per replicate. Rats were fed a basal NFD (control group) containing 0.08 mg/kg chromium (Cr), meanwhile, the basal diets supplemented with 0.2 or 1.0 mg/kg Cr from Cr for 21 days. The results showed that: 1) dietary supplemented with 0.2 mg/kg Cr significantly improved spleen index (P<0.05). 2) Dietary supplemented with 1.0 mg/kg Cr significantly increased the ratio of villous height to crypt depth (P<0.05). 3) Compared with the control group, dietary supplemented with 1.0 mg/kg Cr significantly improved serum albumin and uric acid contents (P<0.05), dietary supplemented with 0.2 mg/kg Cr significantly improved serum uric acid content (P<0.05). It is concluded that under NFD condition, the proper Cr supplementation has some effects on improving immune organs indexes, intestinal mucosa morphology and serum protein metabolism.

Cite this article

ZHENG Cancai , HUANG Yanling . Effects of Chromium Supplemented Level in Nitrogen-Free Diets on Organ Indexes, Intestinal Mucosa Morphology and Serum Parameters of Rats[J]. Chinese Journal of Animal Nutrition, 2016 , 28(1) : 157 -162 . DOI: 10.3969/j.issn.1006-267x.2016.01.021

References

[1] LLOYD K E,FELLNER V,MCLEOD S J,et al.Effects of supplementing dairy cows with chromium propionate on milk and tissue chromium concentrations[J].Journal of Dairy Science,2010,93(10):4774-4780.

[2] LI Y S,ZHU N H,NIU P P,et al.Effects of dietary chromium methionine on growth performance,carcass composition,meat colour and expression of the colour-related gene myoglobin of growing-finishing pigs[J].Asian Australasian Journal of Animal Sciences,2013,26(7):1021-1029.

[3] 李斯丽.蛋氨酸铬作为饲料添加剂在生长肥育猪生产中的应用技术研究[D].硕士学位论文.南宁:广西大学,2013.

[4] 范春玲,兰明慧,周玉龙,等.酵母铬对围产后期奶牛免疫功能和泌乳性能的影响[J].中国畜牧杂志,2013,49(23):36-40.

[5] 李燕.有机铬对热应激肉鸭肠黏膜形态、HSP70 mRNA和抗氧化能力的影响[D].硕士学位论文.武汉:华中农业大学,2013.

[6] CHEN N S C,TSAI A,DYER I A.Effect of chelating agents on chromium absorption in rats[J].Journal of Nutrition,1973,103(8):1182-1186.

[7] EMAMI A,GANJKHANLOU M,ZALI A.Effects of Cr methionine on glucose metabolism,plasma metabolites,meat lipid peroxidation,and tissue chromium in Mahabadi goat kids[J].Biological Trace Element Research,2015,164(1):50-57.

[8] BERNHARD B C,BURDICK N C,RATHMANN R J,et al.Chromium supplementation alters both glucose and lipid metabolism in feedlot cattle during the receiving period[J].Journal of Animal Science,2012,90(13):4857-4865.

[9] WANG M Q,WANG C,DU Y J,et al.Effects of chromium-loaded chitosan nanoparticles on growth,carcass characteristics,pork quality,and lipid metabolism in finishing pigs[J].Livestock Science,2014,161:123-129.

[10] SCHROEDER H A,BALASSA J J,TIPTON I H.Abnormal trace metals in man-chromium[J].Journal of Chronic Diseases,1962,15(10):941-964.

[11] MERTZ W,ROGINSKI E E.Effects of chromium (Ⅲ) supplementation on growth and survival under stress in rats fed low protein diets[J].The Journal of Nutrition,1969,97(4):531-536.

[12] REEVES P G,NIELSEN F H,FAHEY G C,Jr.AIN-93 purified diets for laboratory rodents:final report of the American institute of nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet[J].The Journal of Nutriton,1993,123(11):1939-1951.

[13] BERNAO A,MESEGUER I,AGUILAR M V,et al.Effect of different doses of chromium picolinate on protein metabolism in infant rats[J].Journal of Trace Elements in Medicine and Biology,2004,18(1):33-39.

[14] 辜良英,许梓荣,查龙应,等.不同形式铬对SD大鼠生长性能、胴体组成和血清生化指标的影响[J].动物营养学报,2007,19(3):258-263.

[15] 马得莹,单安山,李群道.中草药添加剂对蛋雏鸡生长性能和免疫功能的影响[J].动物营养学报,2004,16(2):36-40.

[16] 王珏,郑艺梅,金光明,等.铬对热应激下蛋鸡空肠组织结构的影响[J].畜牧与兽医,2003,35(2):3-5.

[17] 王洪阳.铜、锰、铬和硒不同添加量对断奶獭兔生产性能、血清指标及十二指肠发育的影响[D].硕士学位论文.杨凌:西北农林科技大学,2012.

[18] 闫祥洲,张兆红.有机铬对断奶仔猪生长性能和免疫功能的影响[J].河南农业科学,2005(7):90-94.

[19] 张敏红,张卫红,杜荣,等.补铬对高温环境下猪的铬代谢,生理生化反应和生产性能的影响[J].畜牧兽医学报,2000,31(1):1-8.

[20] 侯小强,罗秉坤,张乃生,等.日粮补铬对早期断奶仔猪生产性能,血液理化和应激反应指标的影响[J].畜牧与兽医,2004,36(4):18-20.

[21] 王敏奇,许晓玲,雷剑,等.不同形式三价铬对肥育猪胴体组成和脂肪代谢的影响[J].畜牧兽医学报,2009,40(1):59-65.

[22] MOONSIE-SHAGEER S,MOWAT D N.Effect of level of supplemental chromium on performance,serum constituents,and immune status of stressed feeder calves[J].Journal of Animal Science,1993,71(1):232-238.

[23] 秦健,杜荣,王俊东.酵母铬与L-肉碱互作对肉鸡蛋白质代谢的影响[J].中国家禽,2011,33(3):22-25.

[24] 程玉芳,苗建民,孙黎,等.日粮铬水平对热应激种公鸡免疫功能及蛋白质代谢的影响[J].中国家禽,2011,33(17):19-21.

[25] 程玉芳,赵美林,靳玲品,等.CrCl3对低温条件下种公鸡血清生化指标的影响[J].畜牧与兽医,2012,44(4):56-58.

[26] 陈强,蔡春标,李建生,等.添加烟酸铬对生长肥育猪的生长性能、代谢及组织学的影响[J].福建农业大学学报,2000,29(4):502-507.

[27] ERNEST A K,GUSTAVE K K,SÉRAPHIN K C.Effect of chromium and proteins diets in rats[J].International Journal of Plant,Animal and Environmental Sciences,2012,2(2):1-8.
Outlines

/