The objective of this survey aim to study the mycotoxins contamination of layers feed in southwest area of China. A total of 95 feed samples from different area (Sichuan, Chongqing, Guizhou, Guangxi and Yunnan) of southwest were obtained to determine the contents of aflatoxin B1 (AFB1), zearalenone (ZON), deoxynivalenol (DON) and fumonisin (FB). Enzyme-linked immunosorbent assay (ELISA) was initially used for determination. Then, some samples would be detected by high performance liquid chromatography (HPLC) if the mycotoxins content was beyond 80% of the national limited standard. The results showed as follows:1) the detection rates of AFB1, DON, ZON and FB in layer's feeds were 83.16%, 93.68%, 94.74% and 100.00%, respectively. The over-limit ratio of AFB1 and ZON was 3.16%, respectively, while DON and FB were not observed to exceed the limits. The average content of AFB1, DON and ZON in layer's feed of different province/city was significantly different (P<0.01), with the Chongqing had the highest content of AFB1 (10.9 μg/kg), Sichuan had the highest content of DON (1.07 mg/kg). 2) The average content of four mycotoxins had no significant difference in different feed type (P>0.05). 3) The content of AFB1 of layer's feed was observed to be different in different production scale company (P<0.05), and the small-scale feed company (less than 50 000 t per year) had higher content of AFB1 (6.36 μg/kg). It is concluded that in southwest of China, the contamination of AFB1, DON, ZON and FB are relative low in layer's feed, but the contents of these four mycotoxins are different in different regions and different scale enterprises.
LI Yaling
,
WANG Jianping
,
LI Yun
,
DING Xuemei
,
BAI Shiping
,
ZENG Qiufeng
,
CHENG Chuanmin
,
GAO Qingjun
,
BAI Fan
,
ZHANG Keying
. Mycotoxin Distribution of Layers Feed in Southwest China[J]. Chinese Journal of Animal Nutrition, 2016
, 28(1)
: 172
-181
.
DOI: 10.3969/j.issn.1006-267x.2016.01.023
[1] RODRIGUES I,NAEHRER K.A three-year survey on the worldwide occurrence of mycotoxins in feedstuffs and feed[J].Toxins,2012,4(9):663-675.
[2] DEVEGOWDA G, RAJU M V L N, AFZALI N,et al.Mycotoxin picture worldwide:novel solutions for their counteraction[J].Feed Compounder (United Kingdom),1998,18(6):22-27.
[3] 杜妮.2013年7~12月全国饲料及原料霉菌毒素污染及调查报告[J].国外畜牧学:猪与禽,2014,34(2):45-47.
[4] 赵丽红,马秋刚,李笑樱,等.抽样调查北京地区猪场饲料及饲料原料赭曲霉毒素A污染状况[J].动物营养学报,2012,24(10):1999-2005.
[5] 张自强,柏凡,张克英,等.我国饲料中黄曲霉毒素B1污染的分布规律研究[J].中国畜牧杂志,2009,45(12):27-30.
[6] 敖志刚,陈代文.2006~2007年中国饲料及饲料原料霉菌毒素污染调查报告[J].中国畜牧兽医,2008,35(1):152-156.
[7] 王若军,苗朝华,张振雄,等.中国饲料及饲料原料受霉菌毒素污染的调查报告[J].饲料工业,2003,24(7):53-54.
[8] 王金勇,刘颖莉,关舒.2012年中国饲料和原料霉菌毒素检测报告[J].中国畜牧杂志,2013,49(4):29-34.
[9] 李荣涛,谢刚,付鹏程,等.小麦和玉米中玉米赤霉烯酮污染情况初探(Ⅰ)[J].粮食储藏,2004(5):36-38.
[10] 程传民,柏凡,李云,等.2013年玉米赤霉烯酮在饲料原料中的污染分布规律[J].中国畜牧杂志,2014,50(16):68-72.
[11] 王金勇,刘颖莉.2011年第三季度中国饲料和原料中霉菌毒素污染调查报告[J].今日养猪业,2013(1):30-31.
[12] 黄广明,李肖红,段智勇,等.饲料及饲料原料中霉菌毒素污染状况的分析[J].养猪,2010(6):6-8.
[13] 蒋惠岚,唐承明,段亚丽,等.广西地区配合饲料霉菌毒素污染状况调查研究[J].饲料工业,2011,32(15):59-62.
[14] 王金勇.2011年全球霉菌毒素调查报告[J].中国畜牧杂志,2012,48(14):49-52.
[15] 刘兴玠,尹秀英,李玉伟,等.我国各地区粮食中黄曲霉产毒性能的调查研究[J].中国医学科学院学报,1981,3(4):266-269.
[16] 李瑞芳,韩北忠,陈晶瑜,等.黄曲霉生长预测模型的建立及其在玉米储藏中的应用[J].中国粮油学报,2008,23(3):144-147.
[17] 甄阳光,柏凡,张克英,等.我国主要饲料原料及产品中呕吐毒素污染分布规律研究[J].中国畜牧杂志,2009,45(8):21-24.
[18] 程传民,柏凡,李云,等.2013年小麦类饲料原料中霉菌毒素污染情况调查[J].粮食与饲料工业,2014,12(9):41-46.
[19] 李群伟,李德安,孟宪清,等.影响镰刀菌生长与产毒的基本因素的研究[J].中国地方病学杂志,1998,17(6):355-358.
[20] 雷元培,马秋刚,谢实勇,等.抽样调查北京地区猪场及饲料原料玉米赤霉烯酮污染状况[J].动物营养学报,2012,24(5):905-910.
[21] 何树森,辛又川,刘全秀,等.四川省部份主粮中霉菌及其毒素污染状况调查[J].预防医学情报杂志,1999,15(4):199-201.
[22] OGUNLADE J T, EWUOLA E O, GBORE F A,et al.Growth performance and egg quality traits of laying hens fed graded levels of dietary fumonisin B1[J].IOSR Journal of Environmental Science, Toxicology and Food Technology,2013,3(2):83-88.
