The study was aimed to investigate the optimization and screening of fermentation medium for the recombinant plectasin, and the effects of recombinant plectasin on growth performance, nutrient digestibility and intestinal microflora in weaning pigs. In the present study, the liquid fermentation was used to prepare the recombinant plectasin in a 30 L fermenter by using batch-fermentation process, and subsequently the expression levels of recombinant plectasin with low salt, basal glycerin and basal soluble starch mediums were compared. Thirty healthy piglets (Duroc× Landrace×Yorkshire) weaned at 24 d were selected and randomly allotted to five groups according to their initial BW. The groups consisted of control group (CON group, a basal diet without antibiotic), sulfuric acid colistin group (CS group, basal diet+0.3% sulfuric acid colistin), antimicrobial peptide group (AP group, basal diet+0.2% recombinant plectasin), probiotics group (PB group, basal diet+0.1% probiotics) and joint application group (PPB group, basal diet+0.2% recombinant plectasin+0.1% probiotics). The experiment lasted for 21 days. The results showed that the wet weight of bacteria in low salt group, basal glycerol group and basal soluble starch group reached the highest value at 114 hours of fermentation, which were 450, 402 and 277 g/L, respectively; the concentrations of total protein in supernatant were 0.38, 3.94 and 5.63 g/L, respectively. Animal trial results showed that compared with CON group, average daily feed intake (ADFI), average daily gain (ADG) and ratio of feed to gain (F/G) were significantly increased (P<0.05) and diarrhea incidence tended to decrease in CS and AP groups (0.05<P<0.10). Compared with other groups, the AP group significantly increased the population of Bifidobacteria in ileal digesta (P<0.05). Compared with CON group, the other groups tended to increase the apparent digestibility of energy and dry matter (0.05<P<0.10). The apparent digestibility of phosphorus in PB group was significantly greater than that in the rest four groups (P<0.01). In conclusion, the yield of recombinant plectasin and the performance of piglets are improved by optimizing the growth medium. And the results preliminarily confirm that recombinant plectasin developed by our laboratory can be used as an alternative to antibiotics in animal feed.
LI Yan
,
WAN Jin
,
CHEN Guang
,
CHEN Daiwen
,
YU Bing
,
HE Jun
. Recombinant Plectasin: Screening of Fermentation Medium and Application for Weaning piglets[J]. Chinese Journal of Animal Nutrition, 2016
, 28(1)
: 208
-216
.
DOI: 10.3969/j.issn.1006-267x.2016.01.027
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