Effects of Candida tropicalis and Mulberry Leaf Flavonoids on Nutrient Metabolism and Rumen Fermentation of Calves

YANG Chuntao; DIAO Qiyu; QU Peibin; SI Bingwen; MA Junnan; ZHOU Yucai; TU Yan

doi:10.3969/j.issn.1006-267x.2016.01.029

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 1: 224 - 234

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.01.029

Molecular Nutrition

Effects of Candida tropicalis and Mulberry Leaf Flavonoids on Nutrient Metabolism and Rumen Fermentation of Calves

YANG Chuntao ,
DIAO Qiyu ,
QU Peibin ,
SI Bingwen ,
MA Junnan ,
ZHOU Yucai ,
TU Yan

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1. Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing Key Laboratory for Dairy Cow Nutrition, Beijing 100081, China;
2. Beijing Capital Agribusiness Simplot Agricultural Science and Technology Co., Ltd., Beijing 100081, China

Received date: 2015-07-14

Online published: 2016-01-22

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Abstract

This study was aimed to investigate the effects of Candida tropicalis (CT) and mulberry leaf flavonoids (MLF) on energy and nitrogen metabolism, rumen microbial protein synthesis and rumen fermentation of pre- and post-weaning calves. Forty-eight Chinese Holstein bull calves aged (20±2) days were used and assigned to one of the four groups with 12 calves each. The calves in the control group (CON group) were fed a basal diet which including a milk replacer and a starter in pre-weaning period and the starter in post-weaning period. Calves in CT, MLF and CM groups were fed the basal diet with CT, MLF and the compound of CT and MLF, respectively. The calves were weaned (milk replacer) at 56 days of age and the trial lasted 60 days. The rumen fluid was collected at 28, 42, 56 and 80 days of age, digestion trials were conducted to estimate nutrients metabolism at 35 and 63 days of age, respectively. The results showed as follows: comparing with CT group, metabolizable energy of pre-weaning calves in CM group was significantly higher (P<0.05); compared with CON group, the utilization of nitrogen of post-weaning calves in CM group was significantly increased (P<0.05); nitrogen biological value of pre-weaning calves in MLF group was significantly higher than that in CON and CT groups (P<0.05). Compared with CON group, rumen fluid pH (28 to 80 days of age) and microbial protein content (56 and 80 days of age) in CT group were significantly increased (P<0.05), but the concentration of ammonia nitrogen was not impacted by treatments (P>0.05); total volatile fatty acid concentration and butyrate content in MLF group were significantly higher than those in CON group at 56 days of age (P<0.05); there was an increasing tendency for acetate to propionate ratio in CT and MLF groups compared to that in CON group (P=0.090), and the contents of acetate and valerate were not significantly affected (P>0.05). In conclusion, the supplementation of CT can help to increase rumen fluid pH and microbial protein synthesis for pre-weaning calves; the supplementation of CT and MLF can increase metabolizable energy of diet and nitrogen biological value for pre-weaning calves, decrease fecal energy and total excrete nitrogen, improve metabolizability of gross energy and utilization of nitrogen of post-weaning calves. Furthermore, it also can improve rumen fermentation. Compared with the effects of single using, the combining of CT and MLF is preferable.

Key words： calf; Candida tropicalis; mulberry leaf flavonoids; microbial protein; nutrient metabolism; rumen fermentation

Cite this article

YANG Chuntao , DIAO Qiyu , QU Peibin , SI Bingwen , MA Junnan , ZHOU Yucai , TU Yan . Effects of Candida tropicalis and Mulberry Leaf Flavonoids on Nutrient Metabolism and Rumen Fermentation of Calves[J]. Chinese Journal of Animal Nutrition, 2016 , 28(1) : 224 -234 . DOI: 10.3969/j.issn.1006-267x.2016.01.029

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