The objective of this experiment was to explore the effects of dietary protein level on homing capability and memory related gene expression of Apis mellifera ligustica. Nine colonies of local Apis mellifera ligustica with equal colony population were randomly divided into 3 groups with 3 replicates in each group. Bees in groups Ⅰ, Ⅱ and Ⅲ were fed diets containing 15%, 25%, 35% protein, respectively. In this study, the homing rate of foragers in the 1 and 2 km away after feeding 45 days and the expression levels of three memory related genes [glutamate receptor type A gene (GluRA), N-methyl-D-aspartic acid receptor type 1 gene(Nmdar1) and tyramine receptor type 1 gene (Tyr1)] in different day-old (newly emerged, 10-day-old and 20-day-old) worker bees were detected. The results showed that the homing rates in the 1 and 2 km away in groups Ⅱ and Ⅲ were significantly higher than those in group Ⅰ (P<0.05), while there was no significant difference between groups Ⅱ and Ⅲ (P>0.05). The expression level of GluRA of 10-day-old and 20-day-old worker bees in group Ⅲ was significantly higher than that in group Ⅰ and Ⅱ (P<0.05), and that in group Ⅱ was significantly higher than that in group Ⅰ (P<0.05). The expression level of Nmdar1 of 10-day-old and 20-day-old worker bees in groups Ⅱ and Ⅲ was significantly higher than that in group Ⅰ (P<0.05), but there was no significant difference between groups Ⅱ and Ⅲ (P>0.05). The expression level of Tyr1 of 10-day-old worker bees in groups Ⅱ and Ⅲ was significantly higher than that in group Ⅰ (P<0.05), but there was no significant difference between groups Ⅱ and Ⅲ (P>0.05). Besides that, there were no significant differences in the expression levels of GluRA, Nmdar1 and Tyr1 of newly emerged worker bees among the three groups (P>0.05), and the expression level of Tyr1 of 10-day-old worker bees among the three groups also had no significant difference (P>0.05). In conclusion, the lower level of protein in the diet may infect the homing ability and the expression of memory related genes of Apis mellifera ligustica.
YUAN An
,
GUO Yahui
,
WU Xiaobo
,
HUANG Xiao
,
LIAO Chunhua
. Effects of Dietary Protein Level on Homing Capability and Memory Related Gene Expression of Apis mellifera ligustica[J]. Chinese Journal of Animal Nutrition, 2016
, 28(1)
: 296
-302
.
DOI: 10.3969/j.issn.1006-267x.2016.01.038
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