Effects of Glutamine on Growth Performance, Intestinal Morphology and Non-Specific Immune Related Gene Expression of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)

YE Shazhou; ZHANG Jie; CHEN Haimin; ZHOU Qicun; ZHU Fangzhou

doi:10.3969/j.issn.1006-267x.2016.02.020

2016 , Vol. 28 >Issue 2: 468 - 476

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.02.020

AQUACULTURE NUTRITION

Effects of Glutamine on Growth Performance, Intestinal Morphology and Non-Specific Immune Related Gene Expression of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)

YE Shazhou ,
ZHANG Jie ,
CHEN Haimin ,
ZHOU Qicun ,
ZHU Fangzhou

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1. Key Laboratory of Marine Bioengineering of Zhejiang Province, School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2. Laboratory of Fish Nutrition, School of Marine Sciences, Ningbo University, Ningbo 315211, China;
3. Zhengzhou Xinwei Nutrition Technology Company, Zhengzhou 450100, China

Received date: 2015-08-24

Online published: 2016-02-19

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Abstract

The effects of glutamine (Gln) on growth performance, intestinal morphology and non-specific immune related gene expression of juvenile yellow catfish (Pelteobagrus fulvidraco)were evaluated in this study. Two hundred and forty juvenile yellow catfish with the initial body weight of (2.49±0.04) g were randomly divided into four groups with three replicates, and there were twenty fish in each replicate. Four experimental diets were formulated to contain 0 (control), 0.10%, 0.20% and 0.40% Gln, which were randomly fed one of four groups for 70 days. The results showed that the growth performance indices were not significantly affected by Gln supplementation (P>0.05). Diets supplemented with Gln could improve the intestinal villus development, and increase the villus height and folds depth, but decrease the muscle thickness. Compared with control group, the intestinal villus height and folds depth in 0.20% and 0.40% groups were significantly increased (P<0.05), and the muscle thickness was significantly decreased (P<0.05). For the Gln supplemental level at 0.10% to 0.20%, the mRNA relative expression level of metallothionein (MT) in muscle had the most obvious change, and that in supplementation groups was significantly higher than that in control group (P<0.05); the mRNA relative expression level of glutathione-S-transferase (GST) in intestine got the most obvious change, and that in 0.20% group was significantly higher than that in control group (P<0.05); the mRNA relative expression levels of heppatic bactericidal protein (Hepc) and heat shock cognate protein 70 (Hsc70) both in liver got the most obvious changes, the mRNA relative expression level of Hepc in 0.10% group was over 73 times than that of control group (P<0.05), and the mRNA relative expression level of Hsc70 in 0.20% group was over triple than that of control group (P<0.05). In summary, these results suggest that Gln can improve the non-specific immune and antioxidant ability of juvenile yellow catfish at certain supplemental level.

Key words： yellow catfish (Pelteobagrus fulvidraco); glutamine; non-specific immune; intestine morphology

Cite this article

YE Shazhou , ZHANG Jie , CHEN Haimin , ZHOU Qicun , ZHU Fangzhou . Effects of Glutamine on Growth Performance, Intestinal Morphology and Non-Specific Immune Related Gene Expression of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)[J]. Chinese Journal of Animal Nutrition, 2016 , 28(2) : 468 -476 . DOI: 10.3969/j.issn.1006-267x.2016.02.020

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