FEED SCIENCE AND TECHNOLOGY

Effects of Dietary Bacillus subtilis on Growth Performance, Liver Antioxidant Ability, Intestinal Microflora Structure and Disease Resistance of Juvenile Blunt Snout Bream (Megalobrama amblycephala)

  • SUN Shengming ,
  • SU Yanli ,
  • ZHANG Wuxiao ,
  • GE Xianping ,
  • ZHU Jian
Expand
  • 1. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;
    2. Wuxi Fishery College, Nanjing Agricultural University, Wuxi 214081, China

Received date: 2015-07-24

  Online published: 2016-02-19

Abstract

This experiment was conducted to investigate the effects of dietary Bacillus subtilis (B. subtilis) on growth performance, liver antioxidant ability, intestinal microflora structure and disease resistance of juvenile blunt snout bream (Megalobrama amblycephala). Three hundred and sixty juvenile blunt snout bream with an average body weight of (1.81±0.01) g were randomly chosen and divided into 4 groups with 3 replicates per group and 30 fish per replicate. Four isonitrogenous and isolipidic diets were formulated with the B. subtilis supplemental level was 0 (T0 group, as control group), 2×107 (T1 group), 2×108 (T2 group), 2×109 CFU/g (T3 group), respectively, and those diets were randomly fed one of four groups for 8 weeks. The results showed as follows:1) the weight gain rate (WGR) and specific growth rate (SGR) of T1 and T2 groups were significantly higher than those of control group (P<0.05), and the feed conversation ratio (FCR) of T1 group was significantly lower than that of control group (P<0.05). 2) Compared with the control group, the activities of liver antioxidant enzymes (superoxide dismutase, catalase and catalase) of T1 group were significantly increased (P<0.05), meanwhile, the liver malondialdehyde content in that group was significantly decreased (P<0.05). 3) No significant differences were observed in moisture, crude protein, crude lipid and ash contents of whole body among all groups (P>0.05). 4) The denaturing gradient gel electrophoresis (DGGE) image demonstrated that the intestinal microflora structure was changed when juvenile blunt snout bream fed diets supplemented with different levels of B. subtilis. 5) Following challenged by Aeromonas hydrophila on the 96 h, fish fed different experimental diets showed no significant differences in cumulative mortality, but the cumulative mortality in T1 and T2 groups was lower than that in control and T3 groups. It is concluded that adding 2×107 CFU/g B. subtilis to juvenile blunt snout bream diet can improve growth performance and antioxidant ability, and change the intestinal microflora structure, but cannot significantly affect the body composition and disease resistance.

Cite this article

SUN Shengming , SU Yanli , ZHANG Wuxiao , GE Xianping , ZHU Jian . Effects of Dietary Bacillus subtilis on Growth Performance, Liver Antioxidant Ability, Intestinal Microflora Structure and Disease Resistance of Juvenile Blunt Snout Bream (Megalobrama amblycephala)[J]. Chinese Journal of Animal Nutrition, 2016 , 28(2) : 507 -514 . DOI: 10.3969/j.issn.1006-267x.2016.02.024

References

[1] 孙云章,杨红玲,马如龙,等.斜带石斑鱼消化道乳酸菌在模拟胃肠道环境中的存活[J].中国水产科学,2010,17(1):128-135.
[2] 曾东,王益平,倪学勤,等.鲤益生菌筛选及部分菌株对鲤前肠黏液的体外黏附作用[J].中国水产科学,2009,16(3):427-433.
[3] MERRIFIELD D L,DIMITROGLOU A,FOEY A,et al.The current status and future focus of probiotic and prebiotic applications for salmonids[J].Aquaculture,2010,302(1/2):1-18.
[4] 江永明,付天玺,张丽,等.微生物制剂对奥尼罗非鱼生长及消化酶活性的影响[J].水生生物学报,2011,35(6):998-1004.
[5] 刘晓勇,张颖,齐茜,等.枯草芽孢杆菌对杂交鲟幼鱼生长性能、消化酶活性及非特异性免疫的影响[J].中国水产科学,2011,18(6):1315-1320.
[6] 程远,黄凯,黄秀芸,等.饲料中添加枯草芽孢杆菌对吉富罗非鱼幼鱼生长性能、免疫力和抗氧化功能的影响[J].动物营养学报,2014,26(6):1503-1512.
[7] 殷海成,赵红月,黄进,等.枯草芽孢杆菌对免疫和未免疫黄河鲤免疫功能和抗病力的影响[J].动物营养学报,2013,25(7):1559-1567.
[8] ALY S M,AHMED Y A G,AHAREEB A A A,et al.Studies on Bacillus subtilis and Lactobacillus acidophilus,as potential probiotics,on the immune response and resistance of tilapia nilotica (Oreochromis niloticus) to challenge infections[J].Fish & Shellfish Immunology,2008,25(1/2):128-136.
[9] AI Q H,XU H G,MAI K S,et al.Effects of dietary supplementation of Bacillus subtilis and fructooligosaccharide on growth performance,survival,non-specific immune response and disease resistance of juvenile large yellow croaker,Larimichthys crocea[J].Aquaculture,2011,317(1/2/3/4):155-161.
[10] SHEN W Y,FU L L,LI W F,et al.Effect of dietary supplementation with Bacillus subtilis on the growth,performance,immune response and antioxidant activities of the shrimp (Litopenaeus vannamei)[J].Aquaculture Research,2010,41(11):1691-1698.  
[11] 王为民.团头鲂养殖产业现状[J].科学养鱼,2009(4):44-45.
[12] 蒋阳阳,李向飞,刘文斌,等.不同蛋白质和脂肪水平对1龄团头鲂生长性能和体组成的影响[J].水生生物学报,2012,36(5):826-836.
[13] 李晓,李冰,董玉峰,等.精养团头鲂池塘沉积物微生物群落的结构特征及组成多样性分析[J].水产学报,2014,28(2):218-227.
[14] 孙盛明,朱健,戈贤平,等.零换水条件下养殖水体中碳氮比对生物絮团形成及团头鲂肠道菌群结构的影响[J].动物营养学报,2015,27(3):948-955.
[15] AVELLA M A,OLIVOTTO I,SILVI S,et al.Effect of dietary probiotics on clownfish:a molecular approach to define how lactic acid bacteria modulate development in a marine fish[J].Regulatory,Integrative and Comparative Physiology,2010,298(2):R359-R371.
[16] SUZER C,ÇOBAN D,KAMACI H O,et al.Lactobacillus spp.bacteria as probiotics in gilthead sea bream (Sparus aurata,L.) larvae:effects on growth performance and digestive enzyme activities[J].Aquaculture,2008,280(1/2/3/4):140-145.
[17] 高权新,施兆鸿,彭士明.益生菌在水产养殖中的研究进展[J].海洋渔业,2013,35(3):364-372.
[18] FARAMARZI M,JAFARYAN H,FARAHI A,et al.The effects on growth and survival of probiotic Bacillus spp.fed to Persian sturgeon (Acipencer persicus) larvae[J].Aquaculture,Aquarium,Conservation & Legislation International Journal of the Bioflux Society,2011,4(1):10-14.
[19] MANDIKI S,MILLA S,WANG N,et al.Effects of probiotic bacteria on growth parameters and immune defence in Eurasian perch Perca fluviatilis L.larvae under intensive culture conditions[J].Aquaculture Research,2011,42(5):693-703.  
[20] WANG Y B,LI J R,LIN J.Probiotics in aquaculture:challenges and outlook[J].Aquaculture,2008,281(1/2/3/4):1-4.
[21] HASSAAN M S,SOLTAN M A,GHONEMY M M R.Effect of synbiotics between Bacillus licheniformis and yeast extract on growth,hematological and biochemical indices of the Nile tilapia (Oreochromis niloticus)[J].The Egyptian Journal of Aquatic Research,2014,40(2):199-208.  
[22] ZOKAEIFAR H,BALCÁZAR J L,SAAD C R,et al.Effects of Bacillus subtilis on the growth performance,digestive enzymes,immune gene expression and disease resistance of white shrimp,Litopenaeus vannamei[J].Fish & Shellfish Immunology,2012,33(4):683-689.  
[23] ZHANG Z F,CHO J H,KIM I H.Effects of Bacillus subtilis UBT-MO2 on growth performance,relative immune organ weight,gas concentration in excreta,and intestinal microbial shedding in broiler chickens[J].Livestock Science,2013,55(2/3):343-347.
[24] LIN Y H,SHIE Y Y,SHIAU S Y.Dietary copper requirements of juvenile grouper,Epinephelus malabaricus[J].Aquaculture,2008,274(1):161-165.  
[25] XIE J,LIU B,ZHOU Q L,et al.Effects of anthraquinone extract from rhubarb Rheum officinale Bail on the crowding stress response and growth of common carp Cyprinus carpio var.Jian[J].Aquaculture,2008(1):5-11.
[26] FATTMAN C L,SCHAEFER L M,OURY T D.Extracellular superoxide dismutase in biology and medicine[J].Free Radical Biology and Medicine,2003,35(3):236-256.  
[27] 姚延丹,李谷,陶玲,等.复合人工湿地-池塘养殖生态系统细菌多样性研究[J].环境科学与技术,2011,34(7):50-55.
[28] LI P,DELBERT M,GATLIN III.Dietary brewers yeast and the prebiotic GrobioticTMAE influence growth performance,immune responses and resistance of hybrid striped bass (Morone chrysops×M.saxatilis) to Streptococcus iniae infection[J].Aquaculture,2004,231(1/2/3/4):445-456.
[29] ABDEL-TAWWAB M,ABDEL-RAHMAN A M,ISMAEL N E M.Evaluation of commercial live bakers' yeast,Saccharomyces cerevisiae as a growth and immunity promoter for fry Nile tilapia,Oreochromis niloticus (L.) challenged in situ with Aeromonas hydrophila[J].Aquaculture,2008,280(1/2/3/4):185-189.
[30] TIMMERMAN H M,KONING C J M,MULDER L,et al.Monostrain,multistrain and multispecies probiotics-a comparison of functionality and efficacy[J].International Journal of Food Microbiology,2004,96(3):219-233.  
Outlines

/