This experiment was conducted to study on the mycotoxins contamination of meat-type poultry feeds in southwest area of China. A total of 100 feed samples from different area (Sichuan, Chongqing, Guizhou, Guangxi and Yunnan) of southwest were obtained to determine the contents of aflatoxin B₁ (AFB₁), zearalenone (ZON), deoxynivalenol (DON) and fumonisin (FB). To determine the contents of mycotoxins, enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) were used. The results showed as follows:the detection rates of AFB₁, DON, ZON and FB in meat-type poultry feeds were 89%, 96%, 85% and 94.74%, respectively. The over-limit ratios of AFB₁ and ZON were 18% and 5%, while DON and FB were not observed to exceed the limits. The average contents of AFB₁, DON and ZON in feed of different area were different, Chongqing had the highest average content of AFB₁ (11.76 μg/kg), Sichuan had the highest average content of DON (1.23 mg/kg), and Yunnan had the highest average content of ZON (0.26 mg/kg). The highest average content of AFB₁ was found in duckling starter feed, followed by broiler starter feed and broiler grower feed. The broiler starter feed had the highest average content of DON and FB. The average contents of these four mycotoxins had no difference in different feed type (P>0.05). The small-scale feed company (between 20 000 t and 50 000 t per year) had higher average content of AFB₁, DON and ZON than the big-scale feed company (more than 100 000 t per year), but there were no significant difference (P>0.05). In conclusion, the mycotoxin (AFB₁, DON, ZON and FB) contamination problem is common in southwest of China, with the AFB₁ has the highest over-limit ratio. The contents of these four mycotoxins detected in the layers feed are influenced by different location, feed types and scales of the feed mills.