Effects of Early Weaning on Jenunal and Ileal Excitary Amino Acid Aarrier 1 Expression of Piglets

CUI Yizhe; WANG Qiuju; SU Jing; LI Yue; ZHOU Yaqiang; CHEN Ling

doi:10.3969/j.issn.1006-267x.2016.02.030

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 2: 555 - 563

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.02.030

SHORT COMMUNICATIONS

Effects of Early Weaning on Jenunal and Ileal Excitary Amino Acid Aarrier 1 Expression of Piglets

CUI Yizhe ,
WANG Qiuju ,
SU Jing ,
LI Yue ,
ZHOU Yaqiang ,
CHEN Ling

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1. College of Animal Science and Veterinary, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
2. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China;
3. Heilongjiang Province Animal Epidemic Prevention and Control Center, Harbin 150069, China

Received date: 2015-09-01

Online published: 2016-02-19

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Abstract

This study was conducted to examine the effects of early weaning on adaptive changes in glutamate transporter gene expression in comparison with the suckling counterpart in the pig during the ages of 10 to 20 days. A total of 40 ternary hybrid piglets, with a similar body weight randomly taken from 40 different sows at 10 days of age, were divided into 2 groups. The trial group were 20 suckling piglets randomly obtained from 20 different sow litters, were allowed to continue suckling with their sows as the suckling group for 10 days; the 20 piglets of the weaning group were taken from another 20 different sow litters, moved off-site and weaned on a weaning diet for 10 days according to standard swine industry early weaning practices. At the end of the trial, 12 piglets were randomly taken from each group to remove the jejunum and ileum, and gene expression of excitatory amino acid carrier 1 (EAAC1) and free amino acids content were determined. The results showed that weaning significantly decreased the abundance of the EAAC1 (57 and 73 ku) and glutamate transporter associate protein 3-18 (GTRAP3-18) (50 ku) protein and mRNA expression in jejunum and ileum of piglets (P<0.05). Weaning significantly increased the contents of glutamate and total free amino acids (P<0.05), while the contents of glutamate and total free amino acids in ileum were significantly decreased (P<0.05). In conclusion, early weaning decrease jejunal and ileal EAAC1 and GTRAP3-18 protein expression and this may, in part, is related to early weaned piglets suffered from lack of nutrition leading to intestinal glutamate uptake disorder.

Key words： piglet; weaning; glutamate transporter; EAAC1; small intestine

Cite this article

CUI Yizhe , WANG Qiuju , SU Jing , LI Yue , ZHOU Yaqiang , CHEN Ling . Effects of Early Weaning on Jenunal and Ileal Excitary Amino Acid Aarrier 1 Expression of Piglets[J]. Chinese Journal of Animal Nutrition, 2016 , 28(2) : 555 -563 . DOI: 10.3969/j.issn.1006-267x.2016.02.030

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