Study on Detoxification of Gossypol in Cottonseed Meal by Chemical Methods

WANG Weiwei; ZHOU Tianbing; LI Aike; HAN Fei; SONG Daijun

doi:10.3969/j.issn.1006-267x.2016.02.031

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 2: 564 - 571

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.02.031

SHORT COMMUNICATIONS

Study on Detoxification of Gossypol in Cottonseed Meal by Chemical Methods

WANG Weiwei ,
ZHOU Tianbing ,
LI Aike ,
HAN Fei ,
SONG Daijun

Expand

1. Academy of State Administration of Grain, Beijing 100037, China;
2. College of Animal Science and Technology, Southwest University, Chongqing 400715, China

Received date: 2015-09-01

Online published: 2016-02-19

Fold

Abstract

This study was conducted to explore an optimal chemical method to remove gossypol from cottonseed meal. Test 1, we studied the effects of moisture, temperature and treatment time on detoxification rate of gossypol, to explore an optimal moisture and temperature for detoxification of gossypol. We found that under the condition of 60 to 100℃, 8% to 16% of moisture and 20 min to 8 h of time, the detoxification rate was increased with the increases of moisture, temperature and time. Test 2, we compared the detoxification effects of 10 chemical detoxication reagents, and found that the detoxification effects of ferric sulfate (FeSO₄), copper sulphate (CuSO₄) and hydrogen peroxide solution (H₂O₂) were better, of which detoxification rate were more than 40%. However, the cottonseed meal after treatment of FeSO₄ was turning black with rust taste. So CuSO₄ and H₂O₂ were the best single gossypol detoxicant. Test 3, we investigated the optimal condition for this mixed detoxicant and tested their effects. We observed that the optimum parameters for CuSO₄ and H₂O₂ as mixed detoxicant were 80℃, 16% solvent consumption and 1 h heating. Under this condition, the detoxification rate for either free or bound gossypol was more than 84%. The mixed detoxicant slightly decreased the crude protein content of cottonseed meal (P>0.05), however, it increased the digestibilities of crude protein (P<0.05). In conclusion, moisture, temperature and time are important for chemical decoxification of cottonseed meal. CuSO₄ and H₂O₂ as mixed detoxicant, under control of these 3 conditions (80℃, 16% supplementation of detoxicant and 1 h of close heating), can effectively remove gossypol from cottonseed meal and improve the digestibility of crude protein and bionic digestive energy. Collectively, our study can provide a good theoretical and technical support for detoxificated cottonseed meal processing.

Key words： chemical detoxification; cottonseed meal; crude protein; digestibility; gossypol

Cite this article

WANG Weiwei , ZHOU Tianbing , LI Aike , HAN Fei , SONG Daijun . Study on Detoxification of Gossypol in Cottonseed Meal by Chemical Methods[J]. Chinese Journal of Animal Nutrition, 2016 , 28(2) : 564 -571 . DOI: 10.3969/j.issn.1006-267x.2016.02.031

References

[1] 李爱科.中国蛋白质饲料资源[M].北京:中国农业大学出版社,2013.
[2] 柴慧娟.棉籽饼粕蛋白资源的利用途径[J].粮油食品科技,1994(1):1-4.
[3] ROBINSON P H,GETACHEW G,DE PETERS E J,et al.Influence of variety and storage for up to 22 Days on nutrient composition and gossypol level of pima cottonseed (Gossypium spp.)[J].Animal Feed Science and Technology,2001,91(3/4):149-156.
[4] CALHOUN M C,KUHLMANN S W,BALDWIN JR B C.Assessing the gossypol status of cattle fed cottonseed products[C]//Proceedings of the Pacific Northwest Animal Nutrition Conference.Portland,1995:147A-157A.
[5] MENA H,SANTOS J E P,HUBER J T,et al.The effects of feeding varying amounts of gossypol from whole cottonseed and cottonseed meal in lactating dairy cows[J].Journal of Dairy Science,2001,84(10):2231-2239.

[6] BARRAZA M L,COPPOCK C E,BROOKS K N,et al.Iron sulfate and feed pelleting to detoxify free gossypol in cottonseed diets for dairy cattle[J].Journal of Dairy Science,1991,74(10):3457-3467.

[7] TABATABAI F,GOLIAN A,SALARMOEINI M.Determination and detoxification methods of cottonseed meal gossypol for broiler chicken rations[J].Journal of Agricultural Sciences and Technology,2002,16(1):3-15.
[8] NAGALAKSHMI D,SASTRY V R B,AGRAWAL D K.Detoxification of undecorticated cottonseed meal by various physical and chemical methods[J].Animal Nutrition and Feed Technology,2002,2(2):117-126.
[9] NAGALAKSHMI D,SASTRY V R B,PAWDE A.Rumen fermentation patterns and nutrient digestion in lambs fed cottonseed meal supplemental diets[J].Animal Feed Science and Technology,2003,103(1/4):1-14.
[10] 张嗣炯.棉籽饼粕脱毒的工艺研究和利用[J].中国粮油学报,1997,12(2):26-29.
[11] 戴卫东,卢伯南,钱礼华,等.热碱法脱除游离棉酚的实验研究[J].应用化工,2004,33(6):57-59.
[12] ZHANG W J,XU Z R,ZHAO S H,et al.Development of a microbial fermentation process for detoxification of gossypol in cottonseed meal[J].Animal Feed Science and Technology,2007,135(1/2):176-186.
[13] NOFTSGER S M,HOPKINS B A,DIAZ D E,et al.Effect of whole and expanded-expelled cottonseed on milk yield and blood gossypol[J].Journal of Dairy Science,2000,83(11):2539-2547.

[14] BLAUWIEKEL R,XU S,HARRISON J H,et al.Effect of whole cottonseed,gossypol,and ruminally protected lysine supplementation on milk yield and composition[J].Journal of Dairy Science,1997,80(7):1358-1365.

[15] American Oil Chemists Society.Office and tentative methods[S].2nd ed.[S.l.]:[s.n.],1975.
[16] LEE K J,DABROWSKI K.High-performance liquid chromatographic determination of gossypol and gossypolone enantiomers in fish tissues using simultaneous electrochemical and ultraviolet detectors[J].Journal of Chromatography B,2002,779(2):313-319.

[17] 魏二虹,张文举,刘东军.不同热处理对棉籽饼中棉酚含量的影响[J].石河子大学学报:自然科学版,2010,28(1):52-57.
[18] 王书云,刘良忠,何东平,等.棉粕化学方法脱除棉酚效果的研究[C]//中国粮油学会油脂分会第十七届学术年会暨产品展示会论文集.郑州:中国粮油学会,2008:226-230.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References