Effects of Dietary Proline Supplementation on Reproductive Performance and Plasma Biochemical Parameters of Pregnant Huanjiang Mini-Pigs

ZHA Wei; KONG Xiangfeng; TAN Minjie; GENG Meimei; XU Liwei; YOU Jinming

doi:10.3969/j.issn.1006-267x.2016.02.033

2016 , Vol. 28 >Issue 2: 579 - 584

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.02.033

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Effects of Dietary Proline Supplementation on Reproductive Performance and Plasma Biochemical Parameters of Pregnant Huanjiang Mini-Pigs

ZHA Wei ,
KONG Xiangfeng ,
TAN Minjie ,
GENG Meimei ,
XU Liwei ,
YOU Jinming

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1. Nutrition Feed Development Engineering Center of Jiangxi Province, Key Laboratory of Animal Nutrition in Jiangxi Province, Jiangxi Agricultural University, Nanchang 330045, China;
2. Key Laboratory for Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
3. Research Center of Mini-Pig, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang 547100, China

Received date: 2015-07-28

Online published: 2016-02-19

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Abstract

This experiment was conducted to investigate the effects of proline metabolism on reproductive performance in pregnant Huanjiang mini-pigs and approach its underlying physio-biochemical mechanism. Fourty-eight female Huanjiang mini-pigs were used in this study. After 15-day of service, the animals were randomly assigned into 3 groups with 8 replicates per group and 2 pigs per replicate. The pigs in the three groups were fed the experimental diets supplemented with 0.77% L-alanine (alanine group), 1% L-proline (proline group), or 0.77% L-alanine + 0.0167% 2-difluoromethylornithine (DFMO) (DFMO group) in basal diet, respectively. After 45-day and 70-day of service, one sow per replicate was weighted and killed by bloodletting. Blood samples were collected for analyzing biochemical parameters. The number of corpora luteum, fetus number and fetus weight were observed, by dissecting the sampled sows. The results showed as follows:the litter weight in proline group after 70-day of service was significantly higher compared with the alanine group (P<0.05); after 45-day of service, the plasma glutamate pyruvate transaminase activity and albumn concentration in the proline group were significantly decreased compared with the alanine group (P<0.05). After 70-day of service, the plasma total cholesterol concentrations in the proline group, and high-density lipoprotein and albumin concentrations in DFMO group were significantly decreased compared with 45-day post-service (P<0.05). The other parameters had no significant difference among three groups (P>0.05). It is concluded that dietary supplementation with proline might increase the growth and development of fetus in pregnant sows via altering the metabolism of nitrogen and lipid during pregnancy.

Key words： proline; biochemical parameter; reproductive performance; pregnancy; Huanjiang mini-pig

Cite this article

ZHA Wei , KONG Xiangfeng , TAN Minjie , GENG Meimei , XU Liwei , YOU Jinming . Effects of Dietary Proline Supplementation on Reproductive Performance and Plasma Biochemical Parameters of Pregnant Huanjiang Mini-Pigs[J]. Chinese Journal of Animal Nutrition, 2016 , 28(2) : 579 -584 . DOI: 10.3969/j.issn.1006-267x.2016.02.033

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