Effects of Dietary Fat Level on Energy Metabolism, Serum Biochemical Indices, Liver Fatty Acid Composition and Liver Type Fatty Acid Binding Protein Gene Expression of Silver Foxes During the Winter Fur-Growing Period

ZHANG Ting; LUO Jing; ZHONG Wei; SUN Weili; WANG Zhuo; SUN Haoran; FAN Yanyan; XING Jingya; LI Guangyu

doi:10.3969/j.issn.1006-267x.2016.02.038

2016 , Vol. 28 >Issue 2: 618 - 626

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.02.038

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Effects of Dietary Fat Level on Energy Metabolism, Serum Biochemical Indices, Liver Fatty Acid Composition and Liver Type Fatty Acid Binding Protein Gene Expression of Silver Foxes During the Winter Fur-Growing Period

ZHANG Ting ,
LUO Jing ,
ZHONG Wei ,
SUN Weili ,
WANG Zhuo ,
SUN Haoran ,
FAN Yanyan ,
XING Jingya ,
LI Guangyu

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State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Science, Chinese Academy of Agricultural Science, Changchun 130112, China

Received date: 2015-08-31

Online published: 2016-02-19

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Abstract

This experiment was conducted to study the effects of dietary fat level on energy metabolism, serum biochemical indices, liver fatty acid composition and liver type fatty acid binding protein (L-FABP) gene expression of silver foxes during the winter fur-growing period. Fifty 145-day-old healthy male silver foxes with a similar body weight were randomly divided into 5 groups with 10 replicates per group and 1 fox per replicate, and they were fed experimental diets containing 10%, 12%, 16%, 20% and 24% lipid mixture (soybean oil:chicken oil=50:50), respectively. The fat level in those experimental diets was 12.85% (group A), 14.71% (group B), 18.72% (group C), 22.23% (group D) and 26.11% (group E), respectively. The experiment was 15 days for adaptation and 75 days for trial period. The results showed as follows:1) dietary fat level significantly or extremely significantly affected the daily dry matter intake, daily metabolizable energy (ME) intake, average daily gain and ME/gain of silver foxes during the winter fur-growing period (P<0.05 or P<0.01), but there was no significant effect on gross energy digestibility (P>0.05). 2) Serum triglyceride (TG) and glucose (GLU) levels showed increasing trends with dietary fat level increasing, and the serum TG level in group E was significantly higher than that in groups A and B (P<0.05), while the serum GLU level in group A was significantly lower than that in other groups (P<0.05). There were no significant differences in serum total cholesterol (TC), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C) contents, and alanine transarninase (ALT) and alanine transarninase (AST) activities among groups (P>0.05). 3) Dietary fat level significantly or extremely significantly affected the proportions of liver polyunsaturated fatty acids (PUFA), monounsaturated fatty acids (MUFA) and satisfied fatty acids (SFA) of silver foxes during the winter fur-growing period (P<0.05 or P<0.01). With the dietary fat level increasing, the proportions of liver PUFA and MUFA were increased, while the proportion of liver SFA was decreased. 4) The relative expression level of L-FABP mRNA in liver was up-regulated with dietary fat level increasing, and that in group E was extremely significantly higher than that in groups A, B and C (P<0.01). These results indicate that the high level of fat in the diet does not conducive to the utilization of dietary nutrients for silver foxes during the winter fur-growing period. Increasing dietary fat level can increase the levels of serum TG and GLU, and promote the unsaturated fatty acid deposition in liver of silver foxes during the winter fur-growing period. Increasing dietary fat level can also up-regulate the liver L-FABP mRNA expression of silver foxes during the winter fur-growing period,which lead to promote the transportation of fat, and then reduce the damage to the liver caused by high fat diet.

Key words： silver foxes; fat level; serum biochemical indices; liver; fatty acid composition; liver type fatty acid binding protein

Cite this article

ZHANG Ting , LUO Jing , ZHONG Wei , SUN Weili , WANG Zhuo , SUN Haoran , FAN Yanyan , XING Jingya , LI Guangyu . Effects of Dietary Fat Level on Energy Metabolism, Serum Biochemical Indices, Liver Fatty Acid Composition and Liver Type Fatty Acid Binding Protein Gene Expression of Silver Foxes During the Winter Fur-Growing Period[J]. Chinese Journal of Animal Nutrition, 2016 , 28(2) : 618 -626 . DOI: 10.3969/j.issn.1006-267x.2016.02.038

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