Ruminant Nutrition

Developmental Changes in Bacterial Colonization and Digestive Function in Ileum of Lambs

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  • 1. Key Laboratory for Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha 410125, China;
    2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2015-09-25

  Online published: 2016-03-14

Abstract

The objective of this study was to investigate the developmental changes in bacterial colonization and digestive function in ileum of lambs. Forty four Xiangdong black lambs were used in this trial, and 4 of them were randomly slaughtered at 1, 7 and 14 days of age (nonrumination period), 28 and 42 days of age (transition period), and 56 and 70 days of age (rumination period), respectively. The ileal chymus was sampled to determine acetate content, activities of amylase, cellulase and xylanase, as well as general bacteria number and selected functional bacterial species (Prevotella, methanogen, and cellulolytic and amylolytic bacteria). The results showed as follows: acetate could be detected in ileal chymus of lambs since at 25 days of age, and the content was significantly increased with increasing days of age (P<0.05); the increase of days of age significantly increased the activities of cellulase and xylanase (P<0.05 or P<0.01); the increase of days of age significantly increased general bacterial number and the percentages of Prevotella, Prevotella ruminocula and methanogen (firstly detected at 7 days of age) in general bacterial (P<0.01); cellulolytic (Fibrobacter succinogenes and Ruminococcus flavefaciens) and amylolytic bacteria (Ruminobacter amylophilu) were firstly detected at 28 days of age. In conclusion, the fiber-degrading capacity in ileum of lambs is established at 28 days of age and then gradually achieved; a great number of methanogen can be detected at 7 days of age, and then the number gets stable.

Cite this article

LI Xiaopeng, JIAO Jinzhen, YAN Qiongxian, TAN Zhiliang . Developmental Changes in Bacterial Colonization and Digestive Function in Ileum of Lambs[J]. Chinese Journal of Animal Nutrition, 2016 , 28(3) : 731 -738 . DOI: 10.3969/j.issn.1006-267x.2016.03.013

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