Tolerance Evaluation of Butyl-Hydroxyanisole in Diets of Largemouth Bass (Micropterus salmoides)

YU Lili, XUE Min, WANG Jia, HAN Fang, ZHENG Yinhua, WU Xiufeng, WU Lixin

doi:10.3969/j.issn.1006-267x.2016.03.015

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 3: 747 - 758

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.03.015

Aquaculture Nutrition

Tolerance Evaluation of Butyl-Hydroxyanisole in Diets of Largemouth Bass (Micropterus salmoides)

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1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China;
2. National Aquafeed Safety Assessment Station, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2015-09-15

Online published: 2016-03-14

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Abstract

A 10-week growth trail was conducted to evaluate the tolerance of largemouth bass (Micropterus salmoides) on butyl-hydroxyanisole (BHA) based on the changes of growth performance, plasma biochemical indexes, tissue antioxidant indexes and histology of liver and intestine. Four experimental diets were prepared with BHA supplemental levels at 0 (D0 group, as control group), 150 (D150 group), 300 (D300 group) and 1 500 mg/kg (D1500 group), in which 150 mg/kg was designed as the maximum recommended level, otherwise the 300 and 1 500 mg/kg were 2 and 10 fold levels of the maximum recommended level, respectively.The largemouth bass were chosen as the target animal, and each diet was fed to 6 replicates with 30 largemouth bass with the initial body weight of (6.20±0.01) g. The results showed as follows: fish in D150 group showed the best growth performance, and no significant differences in final average body weight, weight gain rate, special gain rate and feed intake were observed among other groups (P>0.05). The condition factor, hepatosomatic index and viscerasomatic index in all groups had no significant differences (P>0.05). Plasma total cholesterol (TC), triglyceride (TG) contents and alkaline phosphatase (AKP) activity in control group were significantly higher than those in other groups (P<0.05). Plasma high density lipoprotein cholesterol (HDL-C)/TC in D150 and D300 groups was significantly higher than that in control and D1500 groups (P<0.05). Plasma aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities in D1500 group were significantly lower than those in control group (P<0.05), but had no significant differences with those in D150 and D300 groups (P<0.05). Liver superoxide dismutase (SOD) activity in D150 and D300 groups was significantly lower than that in control group (P<0.05), but had no significant difference with that in D1500 group (P<0.05). Supplementation of 1 500 mg/kg BHA could significantly enhance the liver total anti-oxidant ability (T-AOC) (P<0.05), and malondialdehyde (MDA) content in plasma, heart and liver were significantly decreased in each BHA supplemental groups (P<0.05). Different degrees of liver and intestinal histological damage were observed in control, D150 and D1500 groups, but fish fed diets with 150 and 1 500 mg/kg BHA relieved the symptom. The results show that the supplementation of 150 mg/kg BHA can enhance the fat metabolism and antioxidant protection of largemouth bass, and a 10 fold of safety margin is obtained in the present study.

Key words： largemouth bass (Micropterus salmoides); butyl hydroxyanisole; tolerance; growth; blood indexes; antioxidant; histology

Cite this article

YU Lili, XUE Min, WANG Jia, HAN Fang, ZHENG Yinhua, WU Xiufeng, WU Lixin . Tolerance Evaluation of Butyl-Hydroxyanisole in Diets of Largemouth Bass (Micropterus salmoides)[J]. Chinese Journal of Animal Nutrition, 2016 , 28(3) : 747 -758 . DOI: 10.3969/j.issn.1006-267x.2016.03.015

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