Effects of Curcumin on Ileum Mucosal Morphology, Gene Expression of Tight Junction Proteins and Inflammatory Cytokines, and Serum Immunoglobulin Levels of Early Weaning Piglets

XUN Wenjuan, ZHOU Hanlin, HOU Guanyu, CAO Ting, SHI Liguang, ZHAO Chunping

doi:10.3969/j.issn.1006-267x.2016.03.023

2016 , Vol. 28 >Issue 3: 826 - 833

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.03.023

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Effects of Curcumin on Ileum Mucosal Morphology, Gene Expression of Tight Junction Proteins and Inflammatory Cytokines, and Serum Immunoglobulin Levels of Early Weaning Piglets

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1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China;
2. College of Agriculture, Hainan University, Haikou 570228, China

Received date: 2015-09-25

Online published: 2016-03-14

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Abstract

This experiment was conducted to study the effects of curcumin on ileum mucosal morphology, gene expression of tight junction proteins and inflammatory cytokines, and serum immunoglobulin levels of early weaning piglets. Fifty Duroc×Landrace×Large White healthy piglets (male to female ratio was 1 : 1) with similar parity and body weight, weaned at 21 days of age, were randomly allocated into 5 groups with 10 replicates in each group and 1 pig in each replicate. The dietary treatments were the basal diet (control group), and the basal diet supplemented with 50 mg/kg quinocetone, or 200, 300 or 400 mg/kg curcumin (experimental groups). The preliminary trial lasted for 7 days and piglets were orally challenged with enterotoxigenic Escherichia coli on the fourth day. The experiment lasted for 21 days. The results showed as follows: 1) compared with the control group, supplementation with 300 or 400 mg/kg curcumin significantly improved villus height and villus height/crypt depth ratio (V/C) (P<0.05), significantly reduced crypt depth and villus width(P<0.05), significantly increased the relative expression levels of occludin and zonula occluden-1(ZO-1) mRNA (P<0.05), significantly decreased the relative expression levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and toll-like receptor 4 (TLR4) mRNA (P<0.05), and significantly increased interleukin-10 (IL-10) mRNA relative expression level, serum immunoglobulin G (IgG) and immunoglobulin M (IgM) levels (P<0.05). 2) The villus width and TLR4 mRNA relative expression level were significantly decreased by quinocetone supplementation (P<0.05), but the other indicators were not significantly affected compared with the control group (P>0.05). It is suggested that 300 or 400 mg/kg curcumin supplementation was more effective than quinocetone in improving ileum mucosal barrier integrity, morphology, and immune status of weaning piglets.

Key words： curcumin; weaning piglets; ileal mucosal morphology; tight junction protein; inflammatory cytokine; immunoglobulin

Cite this article

XUN Wenjuan, ZHOU Hanlin, HOU Guanyu, CAO Ting, SHI Liguang, ZHAO Chunping . Effects of Curcumin on Ileum Mucosal Morphology, Gene Expression of Tight Junction Proteins and Inflammatory Cytokines, and Serum Immunoglobulin Levels of Early Weaning Piglets[J]. Chinese Journal of Animal Nutrition, 2016 , 28(3) : 826 -833 . DOI: 10.3969/j.issn.1006-267x.2016.03.023

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