Effects of Flaxseed Oil on mRNA Expression of Key Genes in TLR4 and NOD Signaling Pathways in Liver of Piglets after LPS Challenge

CHEN Shaokui, LIU Yulan, WANG Haibo, WANG Xiuying, ZHU Huiling, ZHANG Jing, WANG Shuhui, TU Zhixiao

doi:10.3969/j.issn.1006-267x.2016.03.031

2016 , Vol. 28 >Issue 3: 891 - 898

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.03.031

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Effects of Flaxseed Oil on mRNA Expression of Key Genes in TLR4 and NOD Signaling Pathways in Liver of Piglets after LPS Challenge

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Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2015-08-18

Online published: 2016-03-14

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Abstract

This experiment was conducted to investigate the effects of flaxseed oil on mRNA expression of key genes in Toll-like receptor 4 (TLR4) and nucleotide binding oligomerization domain protein (NOD) signaling pathways in liver of piglets after lipopolysaccharide (LPS) challenge. Twenty four pigs with similar body weight were randomly divided into 4 groups with 6 replicates per group and 1 pig per replicate, and the 4 groups were control group, LPS group, 2.5% flaxseed oil group (2.5% flaxseed oil + LPS), and 5.0% flaxseed oil group (5.0% flaxseed oil + LPS), respectively. The experiment lasted for 21 days. The pigs in the experimental groups were injected intraperitoneally with 100 μg/kg body weight LPS, whereas the pigs in the control group were injected with an equivalent amount of sterile saline. At 4 h post-challenge, the pigs were slaughtered and liver samples were collected. The mRNA expression levels of inflammatory cytokines and the key genes in TLR4 and NOD signaling pathways were detected by real-time PCR. The results showed as follows: 1) after LPS challenge, the mRNA relative expression levels of cyclo-oxygenase 2 (COX2), heat shock protein 70 (HSP70) and tumor necrosis factor-α (TNF-α) in liver were significantly increased (P<0.05). The mRNA relative expression levels of COX2 and TNF-α in 2.5% flaxseed oil group were significantly lower than those in LPS group (P<0.05), and the TNF-α mRNA relative expression level in 5.0% flaxseed oil group was significantly lower than that in LPS group (P<0.05). 2) After LPS challenge, the mRNA relative expression levels of TLR4, myeloid differentiation factor 88 (MyD88), interleukin-1 receptor-associated kinase 1 (IRAK1), NOD1, NOD2, receptor-interacting serine/threonine-protein kinase 2 (RIPK2) and nuclear factor-κB (NF-κB) in liver were significantly increased (P<0.05). The mRNA relative expression levels of NOD1 and NOD2 in 2.5% flaxseed oil group were significantly lower than those in LPS group (P<0.05), and the mRNA relative expression level of RIPK2 had a tendency to decrease control to the LPS group (0.05≤P<0.10).The mRNA relative expression level of NOD2 in 5.0%flaxseed oil group was significantly lower than that in LPS group (P<0.05). These results indicate that LPS challenge induces inflammatory response in piglets, flaxseed oil can alleviate liver inflammatory response via NOD signaling pathway inhibition.

Key words： piglets; flaxseed oil; LPS; liver; TLR4; NOD

Cite this article

CHEN Shaokui, LIU Yulan, WANG Haibo, WANG Xiuying, ZHU Huiling, ZHANG Jing, WANG Shuhui, TU Zhixiao . Effects of Flaxseed Oil on mRNA Expression of Key Genes in TLR4 and NOD Signaling Pathways in Liver of Piglets after LPS Challenge[J]. Chinese Journal of Animal Nutrition, 2016 , 28(3) : 891 -898 . DOI: 10.3969/j.issn.1006-267x.2016.03.031

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