Short Communications

Effects of Different Stocking Density on Microbial Flora of Duodenum for Caged Layer

Expand
  • College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319

Received date: 2015-09-09

  Online published: 2016-03-14

Abstract

The test used the polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) methods to explore the effects of different stocking density on the microbial flora of duodenum for caged Hy-line Gray laying hens. A total of 1 150 eleven-week-old Hy-line Gray laying hens were randomly allocated into 5 groups with 50 replications with the stocking density of 900, 675, 540, 450 and 380 cm2 per layer, respectively. All the hens were reared under the same environment condition and with the same management model. Five hens were selected randomly from every group at the age of 16, 26 and 50 weeks, the intestinal samples of duodenum were taken to analyze the micro by PCR-DGGE. The results showed that in the same stocking density, the similarity coefficient of duodenum microbial flora of caged layers was decreased with the increasing of breeding weeks; at the 16 week of age, the microbial flora of duodenum was highly similar between high density group and low density group, there was no obvious difference for structure of intestinal flora; at the 26 and 50 week of age, the microbial flora of duodenum was lowly similar between high density group and low density group, there was significant differences for structure of intestinal flora. In conclusion, with the increasing of feeding week age, the structure of the microbial flora of duodenum is changed significantly; two kinds of beneficial bacteria (Lactobacillus gastricus and Lactobacillus alvi) disappear in high stocking density (group D and group E); the feeding density higher than 450 cm2/per layer have adverse effects on the structure of the microbial flora of duodenum of cage laying hens.

Cite this article

YU Jiangming, WANG Qiuju, LIU Bolin, NING Bolin, WANG Long, ZHAO Qianyu, LIU Shengjun, QUAN Jiahui . Effects of Different Stocking Density on Microbial Flora of Duodenum for Caged Layer[J]. Chinese Journal of Animal Nutrition, 2016 , 28(3) : 899 -907 . DOI: 10.3969/j.issn.1006-267x.2016.03.032

References

[1] ONBASILAR E E,AKSOY F T.Stress parameters and immune response of layers under different cage floor and density conditions[J].Livestock Production Science,2005,95(3):255-263.  

[2] 王龙.饲养密度对层叠笼养蛋鸡生产性能以及福利影响[D].硕士学位论文.大庆:黑龙江八一农垦大学,2015:25-29.

[3] SOHAIL M U,HUME M E,BYRD J A,et al.Molecular analysis of the caecal and tracheal microbiome of heat-stressed broilers supplemented with prebiotic and probiotic[J].Avian Pathology,2015,44(2):67-74.  

[4] ROBERTS T,WILSON J,GUTHRIE A,et al.New issues and science in broiler chicken intestinal health:intestinal microbial composition,shifts,and impacts[J].World's Poultry Science Journal,2015,71(2):259-270.  

[5] GONG J H,SI W D,FORSTER R J,et al.16S rRNA gene-based analysis of mucosa-associated bacterial community and phylogeny in the chicken gastrointestinal tracts:from crops to ceca[J].FEMS Microbiology Ecology,2007,59(1):147-157.  

[6] JANCZYK P,HALLE B,SOUFFRANT W.Microbial community composition of the crop and ceca contents of laying hens fed diets supplemented with Chlorella vulgaris[J].Poultry Science,2009,88(11):2324-2332.  

[7] KIM G B,SEO Y M,KIM C H,et al.Effect of dietary prebiotic supplementation on the performance,intestinal microflora,and immune response of broilers[J].Poultry Science,2011,90(1):75-82.  

[8] GABRIEL I,LESSIRE M,MALLET S,et al.Microflora of the digestive tract:critical factors and consequences for poultry[J].World's Poultry Science Journal,2006,62(3):499-511.

[9] KNARREBORG A,SIMON M A,ENGBERG R M,et al.Effects of dietary fat source and subtherapeutic levels of antibiotic on the bacterial community in the ileum of broiler chickens at various ages[J].Applied and Environmental Microbiology,2002,68(12):5918-5924.  

[10] SUZUKI K,KODAMA Y,MITSUOKA T.Stress and intestinal flora[J].Bifidobacteria and Microflora,1989,8(1):23-38.  

[11] ENDO A,OKADA S,MORITA H.Molecular profiling of Lactobacillus,Streptococcus,and Bifidobacterium species in feces of active racehorses[J].Journal of General and Applied Microbiology,2007,53(3):191-200.  

[12] ENDO A,ROOS S,SATOH E,et al.Lactobacillus equigenerosi sp.nov.,a coccoid species isolated from faeces of thoroughbred racehorses[J].International Journal of Systematic and Evolutionary Microbiology,2008,58(4):914-918.  

[13] BORST L B, SUYEMOTO M M,SHIVARAMU K,et al.A chicken embryo lethality assay for pathogenic Enterococcus cecorum[J].Avian Diseases,2014,58(2):244-248.  

[14] KENSE M J,LANDMAN W J M.Enterococcus cecorum infections in broiler breeders and their offspring:molecular epidemiology[J].Avian Pathology,2011,40(6):603-612.  

[15] BOERLIN P,NICHOLSON V,BRASH M,et al.Diversity of Enterococcus cecorum from chickens[J].Veterinary Microbiology,2012,157(3/4):405-411.

[16] CÁRDENAS N,MARTÍN V,DELGADO S,et al.Characterisation of Lactobacillus gastricus strains isolated from human milk[J].International Dairy Journal,2014,39(1):167-177.  

[17] MARTÍN V,CÁRDENAS N,JIMÉNEZ E,et al.Genome sequence of Lactobacillus gastricus PS3,a strain isolated from human milk[J].Genome Announcements,2013,1(4): e00489-13,doi: 10.1128/genomeA.00489-13..

[18] KIM H J,EOM S J,PARK S J,et al.Lactobacillus alvi sp.nov.,isolated from the intestinal tract of chicken[J].FEMS Microbiology Letters,2011,323(1):83-87.  

[19] PANDA A K,REDDY M R,RAMARAO S V,et al.Effect of dietary supplementation of probiotic on performance and immune response of layers in the decline phase of production[J].Indian Journal of Poultry Science,2000,35(1):102-104.

[20] 倪学勤,GONG J,YU H,等.采用PCR-DGGE技术分析蛋鸡肠道细菌种群结构及多样性[J].畜牧兽医学报,2008,39(7):955-961.

[21] 倪学勤,GONG J,YU H,等.PCR-DGGE技术分析蛋鸡MHC基因对肠道细菌种群结构的影响[J].中国农业科学,2009,42(7):2564-2571.

[22] 李永洙,CUI Y Q.利用PCR-DGGE方法分析不同鸡群的盲肠微生物菌群结构变化[J].生态学报,2011,31(21):6513-6521.

[23] 王秋菊,崔一喆,刘胜军,等.笼养海兰褐蛋雏鸡肠道菌群的聚合酶链式反应—变性梯度凝胶电泳分析[J].动物营养学报,2014,26(2):504-512.
Outlines

/