Thyrotropin Receptor Gene Cloning and Expression Response to Dietary Potassium Iodide Addition of Yellow Catfish (Pelteobagrus fulvidraco)

WANG Chunling, ZHANG Hongta, GAO Youling, QIAN Guoying

doi:10.3969/j.issn.1006-267x.2016.03.037

2016 , Vol. 28 >Issue 3: 940 - 952

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.03.037

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Thyrotropin Receptor Gene Cloning and Expression Response to Dietary Potassium Iodide Addition of Yellow Catfish (Pelteobagrus fulvidraco)

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1. College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China;
2. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China

Received date: 2015-09-15

Online published: 2016-03-14

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Abstract

The aims of the present study were to characterize the cDNA full-long sequence of thyrotropin receptor (TSHR) gene of yellow catfish (Pelteobagrus fulvidraco), and to determine the differential expression of TSHR gene in different tissues. In addition, the effects of dietary potassium iodide (KI) addition on TSHR gene expression and growth performance of yellow catfish were revealed. The rapid amplification of cDNA ends (RACE) technique was used to clone the cDNA full-long sequence of TSHR gene. Moreover, RT-qPCR technique was used to determine the relative expression level of TSHR gene. The KI adding levels were set to be 0 (control), 10, 50 and 100 mg/kg in four experimental diets, which were fed to yellow catfish for 27 days. The results showed as follows: the cDNA full-length sequence of TSHR gene was obtained, and it was 2 786 bp, including a complete open reading frame of 2 238 bp encoded a 745 amino acid residues. Blast online analysis indicated that the amino acid sequence of yellow catfish displayed 60% to 87% identity as compared with other known fish species. The analysis results of tissue differential expression of TSHR gene indicated that the highest relative expression level was in thyroid, followed by liver, muscle and intestine. The results of feeding trial were showed that the relative expression level of TSHR gene in thyroid in 100 mg/kg group was significantly higher than that in other groups (P<0.05), and the weight gain, specific growth rate and feed conversion ratio in 50 and 100 mg/kg groups were significantly higher than those in control group (P<0.05). The results in this study imply that the dietary KI in the appropriate amount not only affects the expression of TSHR gene, but also promote the growth of yellow catfish effectively.

Key words： yellow catfish (Pelteobagrus fulvidraco); potasium iodide; thyrotropin receptor; gene clone; gene expression

Cite this article

WANG Chunling, ZHANG Hongta, GAO Youling, QIAN Guoying . Thyrotropin Receptor Gene Cloning and Expression Response to Dietary Potassium Iodide Addition of Yellow Catfish (Pelteobagrus fulvidraco)[J]. Chinese Journal of Animal Nutrition, 2016 , 28(3) : 940 -952 . DOI: 10.3969/j.issn.1006-267x.2016.03.037

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