MOLECULAR NUTRITION

Recombinant Expression and Biological Property Analysis of Goose Avian β-Defensin 7 Protein

  • GAO Mengying ,
  • XU Qianqian ,
  • ZHANG Tingting ,
  • CHEN Yuqiu ,
  • ZHAO Wenjun ,
  • QI Tianming ,
  • MA Deying
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  • College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2015-11-10

  Online published: 2016-04-11

Abstract

The objective of the study was to investigate the biological property of goose avian β-defensin(AvBD) 7. The gene of AvBD7 was cloned into EcoRⅠ and XhoⅠ sites of pProEX HTa vector to construct recombinant plasmid pProEX-AvBD7. The recombinant plasmid was transformed into Escherichia coli Rosetta and the bacteria was induced with isopropy-β-D-thiogalactoside(IPTG). It was analyzed by tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis(Tricine-SDS-PAGE) that the recombinant protein size was 10 to 15 ku, which was the same as the expected size. Antimicrobial activity of the recombinant fusion protein against Escherichia coli, Salmonella pullorum, Staphylococcus aureus, Micrococcus tetragenus and Bacillus subtilis was measured in vitro. In addition, the effect of ionic strength on the antibacterial activity and hemolytic activity of the recombinant fusion protein were investigated. The results showed that the recombinant goose AvBD7 protein exhibited significant antimicrobial activity against the five bacteria investigated(P<0.05), and antibacterial activity enhanced with the increasing of protein concentration. The antibacterial activity of recombinant goose AvBD7 protein was significantly inhibited by high salt concentration(150 mmol/L)(P<0.05). In addition, recombinant goose AvBD7 protein showed little hemolytic activity against chicken erythrocytes(P>0.05). In conclusion, recombinant goose AvBD7 protein exhibits extensive antimicrobial activity. High salt concentration significantly decrease its antibacterial activity. In addition, the recombinant goose AvBD7 protein shows little hemolytic activity.

Cite this article

GAO Mengying , XU Qianqian , ZHANG Tingting , CHEN Yuqiu , ZHAO Wenjun , QI Tianming , MA Deying . Recombinant Expression and Biological Property Analysis of Goose Avian β-Defensin 7 Protein[J]. Chinese Journal of Animal Nutrition, 2016 , 28(4) : 1114 -1121 . DOI: 10.3969/j.issn.1006-267x.2016.04.020

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