Effects of Different Dietary Crude Protein Levels on Amino Acid Flows and Absorbability in Small Intestine of Growing Lambs

NIU Lu; YIN Hua; ZHENG Meng; GAN Mailin; CHEN Aodong; LIU Chenli; CHEN Daofu; GAO Wei

doi:10.3969/j.issn.1006-267x.2016.04.036

2016 , Vol. 28 >Issue 4: 1241 - 1249

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.04.036

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Effects of Different Dietary Crude Protein Levels on Amino Acid Flows and Absorbability in Small Intestine of Growing Lambs

NIU Lu ,
YIN Hua ,
ZHENG Meng ,
GAN Mailin ,
CHEN Aodong ,
LIU Chenli ,
CHEN Daofu ,
GAO Wei

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College of Animal Science & Technology, Shihezi University, Shihezi 832003, China

Received date: 2015-11-10

Online published: 2016-04-11

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Abstract

This experiment was conducted to compare the effects of different dietary crude protein(CP) levels on amino acid(AA) flows and absorbability in small intestine of growing lambs. Three Kazakh male lambs [(30.0±3.7) kg of body weight] fitted with permanent ruminal, duodenal and ileal fistulas were selected. A 3×3 Latin square design was adopted. Dietary CP levels of different treatments were 11.00%, 12.00% and 13.00%, respectively; the pretest lasted for 10 days, and the test lasted for 10 days. A dual-phase marker system with lithium-chromium ethylenediamine tetra-acetate(LiCr-EDTA) and ytterbium-actinium(Yb-Ac) as liquid-phase and particulate-phase digesta flow makers was adopted to measure small intestinal digesta flows, respectively. The results showed as follows:the intake, apparent digestibility and retention of nitrogen were increased with increasing level of dietary CP, and 13% treatment was significantly higher than 11% treatment(P<0.05); in addition, there were increasing trends of apparent digestibility of dry matter, organic matter and neutral detergent fiber(0.05≤P <0.1), but no significant effects on apparent digestibility of acidic detergent fiber was found(P>0.05). Although intestinal true digesta flows and amino acid absorbability were not significantly affected by the increasing dietary CP level(P>0.05), but the flows of nitrogen and total amino acids, as well as absorption rates of essential amino acids, such as arginine, hisitidine and lysine, were numerically increased. The results indicate that increasing dietary CP level has the potential to improve digestibility, intestinal digesta flows and amino acid absorption rate.

Key words： rumen-degradable protein; amino acid; flow; absorbability; Kazakh lambs

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NIU Lu , YIN Hua , ZHENG Meng , GAN Mailin , CHEN Aodong , LIU Chenli , CHEN Daofu , GAO Wei . Effects of Different Dietary Crude Protein Levels on Amino Acid Flows and Absorbability in Small Intestine of Growing Lambs[J]. Chinese Journal of Animal Nutrition, 2016 , 28(4) : 1241 -1249 . DOI: 10.3969/j.issn.1006-267x.2016.04.036

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