Effects of Dietary Gracilaria lemaneiformis on Growth Performance, Serum Biochemical Indices and Muscle Fatty Acid Composition of Grass Carp(Ctenopharyngodon idellus)

CHEN Ming; LIU Yongjian; LI Yating; CHEN Xianquan; ZENG Shuailin; TIAN Lixia

doi:10.3969/j.issn.1006-267x.2016.04.038

2016 , Vol. 28 >Issue 4: 1257 - 1266

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.04.038

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Effects of Dietary Gracilaria lemaneiformis on Growth Performance, Serum Biochemical Indices and Muscle Fatty Acid Composition of Grass Carp(Ctenopharyngodon idellus)

CHEN Ming ,
LIU Yongjian ,
LI Yating ,
CHEN Xianquan ,
ZENG Shuailin ,
TIAN Lixia

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Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China

Received date: 2015-10-07

Online published: 2016-04-11

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Abstract

In order to evaluate the effects of dietary Gracilaria lemaneiformis on growth performance, serum biochemical indices and muscle fatty acid composition of grass carp(Ctenopharyngodon idellus), juvenile fish at the average body weight of(15.94±0.10) g were fed six isonitrogenous and isolipid diets containing graded levels of Gracilaria lemaneiformis [0(control group), 1%, 2%, 3%, 4% and 5%, respectively] for 8 weeks. Each diet fed four water tanks and each tank had 30 fish. The results showed as follows:1) there were no significant differences in weight gain rate(WGR), specific growth ratio(SGR), survival rate(SR) and feed efficiency(FE) of fish fed diets supplemented with different proportions of Gracilaria lemaneiformis(P>0.05). 2) Hepato-somatic index(HSI) of fish in experimental groups was higher than that in control group, and the differences were significant except 5% supplementation group(P<0.05). 3) Crude protein content in whole body, liver and muscle was not significantly affected by diets supplemented with different levels of Gracilaria lemaneiformis(P>0.05), however, there were significant differences in the contents of ash and crude lipid in whole body, crude lipid in liver and moisture in muscle(P<0.05). 4) Total protein(TP), total cholesterol(TCHO), triglycerides(TG) and low density lipoprotein(LDL) contents in serum in 5% supplementation group had the lowest values, and significantly higher than those in control group(P<0.05), but no significant differences were found between control group and other experimental groups(P>0.05). 5) Fatty acid composition in muscle of grass carp was affected by diets with different proportions of Gracilaria lemaneiformis. Diets supplemented with different proportions of Gracilaria lemaneiformis could significantly affect the contents of fatty acids except C14:1, C20:0 and C20:2 in muscle(P<0.05), and the highest values of polyunsaturated fatty acids(PUFA) content and n-3/n-6 PUFA were found in 3% supplementation group. The contents of C20:5(EPA), C22:5(DPA), C22:6(DHA) and EPA+DHA in muscle in 1%, 2%, 3%, 4% and 5% supplementation groups were significantly higher than those in control group(P<0.05). The present results indicate that diets supplemented with a certain proportion of Gracilaria lemaneiformis have no significant effect on the growth of grass carp, but can reduce the blood lipid contents. It can also influence the fatty acid composition in muscle, improve the functional fatty acid contents, such as DHA and EPA, and increase the content of PUFA. The fish fed with 3% Gracilaria lemaneiformis can better increase the functional fatty acid contents was, and the fish fed with 5% Gracilaria lemaneiformis have a relatively better growth performance as well as an obviously lower blood lipid.

Key words： Gracilaria lemaneiformison; grass carp(Ctenopharyngodon idellus); growth performance; serum biochemical indices; muscle fatty acids

Cite this article

CHEN Ming , LIU Yongjian , LI Yating , CHEN Xianquan , ZENG Shuailin , TIAN Lixia . Effects of Dietary Gracilaria lemaneiformis on Growth Performance, Serum Biochemical Indices and Muscle Fatty Acid Composition of Grass Carp(Ctenopharyngodon idellus)[J]. Chinese Journal of Animal Nutrition, 2016 , 28(4) : 1257 -1266 . DOI: 10.3969/j.issn.1006-267x.2016.04.038

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