Effects of Weaner Time on Rumen Morphology and Gene Expressions Involved in Rumen Epidermis Growth of Hu Lambs at Different Days of Age

LIU Ting; LI Fadi; LI Chong; WANG Weimin; WANG Xiaojuan; LI Fei; ZHENG Chen; MO Futao; WANG Fangbin; LA Yongfu; LI Baosheng

doi:10.3969/j.issn.1006-267x.2016.05.014

2016 , Vol. 28 >Issue 5: 1384 - 1393

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.05.014

RUMINANT NUTRITION

Effects of Weaner Time on Rumen Morphology and Gene Expressions Involved in Rumen Epidermis Growth of Hu Lambs at Different Days of Age

LIU Ting ,
LI Fadi ,
LI Chong ,
WANG Weimin ,
WANG Xiaojuan ,
LI Fei ,
ZHENG Chen ,
MO Futao ,
WANG Fangbin ,
LA Yongfu ,
LI Baosheng

Expand

1. College of Animal Science and Technology, Gansu Agriculture University, Lanzhou 730070, China;
2. Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin 733300, China;
3. College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
4. Jinchang Zhongtian Sheep Industry Co. Ltd., Jinchang 737100, China

Received date: 2016-01-12

Online published: 2016-05-24

Fold

Abstract

The objective of this study was to investigate the effects of weaner time on rumen morphology and gene expressions involved in rumen epidermis growth of Hu lambs at different days of age. Two-factor design was used, and the factors were weaner time and days of age of lambs. A total of 54Hu lambs with similar birth weight[(3.51±0.57) kg] were selected, and 6 of them were randomly selected and slaughtered on 28 days of age, while the remaining animals were randomly assigned to a 28 days of age weaner group[(8.21±0.97) kg] or a 56 days of age weaner group[(8.06±0.53) kg]. Six lambs from each group were randomly selected and slaughtered at 42, 56, 70 and 84 days of age, respectively. The ventral sac sample of rumen was collected to measure rumen papilla height, width and muscular thickness, and the tissue sample was extracted total RNA to measure gene expressions involved in rumen epidermis growth. The results showed as follows:rumen papilla height and thickness in 28 days of age weaner group was significantly higher than that in 56 days of age weaner group (P<0.05), however, rumen muscular thickness in 28 days of age weaner group was significantly lower than that in 56 days of age weaner group (P<0.05). There were significant interactions of weaner time and days of age for rumen papilla height and muscular thickness (P<0.05). The expressions of insulin-like growth factor binding protein (IGFBP)3, IGFBP5 and IGFBP6 in 28 days of age weaner group were significantly higher compared with 56 days of age weaner group (P<0.05). Rumen papilla length was negatively correlated with expressions of IGFBP6 and transforming growth factor β (TGFβ)1(r=-0.318, P=0.001; r=-0.520, P<0.001); rumen papilla width was negatively correlated with the expression of TGFβ1 (r=-0.275, P=0.004), while were positively correlated with expressions of IGFBP3 and IGFBP5 (r=0.344, P<0.001; r=0.256, P=0.001). In conclusion, the results indicate that weaning at 28 days of age can promote rumen papillae development, affect expressions of genes involved in rumen epidermis growth, and may modulate early development of rumen of Hu lambs.

Key words： Hu lamb; early weaning; rumen morphology; development; gene expression

Cite this article

LIU Ting , LI Fadi , LI Chong , WANG Weimin , WANG Xiaojuan , LI Fei , ZHENG Chen , MO Futao , WANG Fangbin , LA Yongfu , LI Baosheng . Effects of Weaner Time on Rumen Morphology and Gene Expressions Involved in Rumen Epidermis Growth of Hu Lambs at Different Days of Age[J]. Chinese Journal of Animal Nutrition, 2016 , 28(5) : 1384 -1393 . DOI: 10.3969/j.issn.1006-267x.2016.05.014

References

[1] LESMEISTER K E,HEINRICHS A J, GABLER M T.Effects of supplemental yeast (Saccharomyces cerevisiae) culture on rumen development,growth characteristics,and blood parameters in neonatal dairy calves[J].Journal of Dairy Science,2004,87(6):1832-1839.

[2] RUSSELL J B,O'CONNOR J D,FOX D G,et al.A net carbohydrate and protein system for evaluating cattle diets:Ⅰ.Ruminal fermentation[J].Journal of Animal Science,1992,70(11):3551-3561.
[3] LESMEISTER K E,HEINRICHS A J.Effects of adding extra molasses to a texturized calf starter on rumen development,growth characteristics,and blood parameters in neonatal dairy calves[J].Journal of Dairy Science,2005,88(1):411-418.

[4] ŽITÑAN R,VOIGT J,WEGNER J,et al.Morphological and functional development of the rumen in the calf:influence of the time of weaning.1.Morphological development of rumen mucosa[J].Archiv für Tierernaehrung,1999,52(4):351-362.
[5] KLEIN R D,KINCAID R L,HODGSON A S,et al.Dietary fiber and early weaning on growth and rumen development of calves[J].Journal of Dairy Science,1987,70(10):2095-2104.

[6] 李述信,孟广政,于建国,等.AOAC分析方法手册[M].14版.北京:中国光学学会光谱专业委员会,1986.
[7] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007.
[8] 中国饲料数据库.中国饲料成分及营养价值表(2013年第24版)(续)[J].中国饲料,2013(22):38-42.
[9] 马志远,李飞,李发弟,等.早期断奶对湖羊羔羊生长性能及胃肠道发育的影响[J].动物营养学报,2015,27(5):1385-1393.
[10] 施恩青.改良与常规石蜡切片方法在切片质量方面的比较[J].齐齐哈尔医学院学报,2014,35(16):2442-2443.
[11] SCHMITTGEN T D,LIVAK K J.Analyzing real-time PCR data by the comparative C_T method[J].Nature Protocols,2008,3(6):1101-1108.

[12] SHI Y G,MASSAGUÉ J.Mechanisms of TGF-β signaling from cell membrane to the nucleus[J].Cell,2003,113(6):685-700.

[13] KRISHNAN K,ARNONE B,BUCHMAN A.Intestinal growth factors:potential use in the treatment of inflammatory bowel disease and their role in mucosal healing[J].Inflammatory Bowel Diseases,2011,17(1):410-422.

[14] CONNOR E E,BALDWIN Ⅵ R L,WALKER M P,et al.Transcriptional regulators transforming growth factor-β₁ and estrogen-related receptor-α identified as putative mediators of calf rumen epithelial tissue development and function during weaning[J].Journal of Dairy Science,2014,97(7):4193-4207.

[15] NAEEM A,DRACKLEY J K,LANIER J S,et al.Ruminal epithelium transcriptome dynamics in response to plane of nutrition and age in young Holstein calves[J].Functional & Integrative Genomics,2014,14(1):261-273.

[16] FIRTH S M,BAXTER R C.Cellular actions of the insulin-like growth factor binding proteins[J].Endocrine Reviews,2002,23(6):824-854.

[17] SANDERSON I R.Short chain fatty acid regulation of signaling genes expressed by the intestinal epithelium[J].The Journal of Nutrition,2004,134(9):2450S-2454S.
[18] MALHI M,GUI H B,YAO L,et al.Increased papillae growth and enhanced short-chain fatty acid absorption in the rumen of goats are associated with transient increases in cyclin D₁ expression after ruminal butyrate infusion[J].Journal of Dairy Science,2013,96(12):7603-7616.

[19] STEELE M A,CROOM J,KAHLER M,et al.Bovine rumen epithelium undergoes rapid structural adaptations during grain-induced subacute ruminal acidosis[J].American Journal of Physiology Regulatory,Integrative and Comparative Physiology,2011,300(6):R1515-R1523.
[20] BACH L A.IGFBP-6 five years on; not so ‘forgotten’?[J].Growth Hormone & IGF Research,2005,15(3):185-192.

[21] STEELE M A,SCHIESTEL C,ALZAHAL O,et al.The periparturient period is associated with structural and transcriptomic adaptations of rumen papillae in dairy cattle[J].Journal of Dairy Science,2015,98(4):2583-2595
[22] LESMEISTER K E,TOZER P R,HEINRICHS A J.Development and analysis of a rumen tissue sampling procedure[J].Journal of Dairy Science,2004,87(5):1336-1344.

[23] GORKA P,KOWALSKI Z M,PIETRZAK P,et al.Effect of sodium butyrate supplementation in milk replacer and starter diet on rumen development in calves[J].Journal of Physiology and Pharmacology,2009,60(Suppl.3):47-53.
[24] REY M,ENJALBERT F, MONTEILS V.Establishment of ruminal enzyme activities and fermentation capacity in dairy calves from birth through weaning[J].Journal of Dairy Science,2012,95(3):1500-1512.

[25] ANDERSON K L,NAGARAJA T G,MORRILL J L.Ruminal metabolic development in calves weaned conventionally or early[J].Journal of Dairy Science,1987,70(5):1000-1005.

[26] SUÁREZ B J,VAN REENEN C G,STOCKHOFE N,et al.Effect of roughage source and roughage to concentrate ratio on animal performance and rumen development in veal calves[J].Journal of Dairy Science,2007,90(5):2390-2403.

[27] BEIRANVAND H,GHORBANI G R,KHORVASH M,et al.Forage and sugar in dairy calves' starter diet and their interaction on performance,weaning age and rumen fermentation[J].Journal of Animal Physiology and Animal Nutrition,2014,98(3):439-445.

[28] NEMATI M,AMANLOU H,KHORVASH M,et al.Rumen fermentation,blood metabolites,and growth performance of calves during transition from liquid to solid feed:effects of dietary level and particle size of alfalfa hay[J].Journal of Dairy Science,2015,98(10):7131-7141.

[29] STOBO I J,ROY J H,GASTON H J.Rumen development in the calf.1.The effect of diets containing different proportions of concentrates to hay on rumen development[J].The British Journal of Nutrition,1966,20(2):171-188.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References