Dietary Arginine Requirement of Genetically Improved Farmed Tilapia (Oreochromis niloticus)

WU Wenyi; JIANG Ming; LIU Wei; WU Fan; TIAN Juan; YANG Changgeng; WEN Hua

doi:10.3969/j.issn.1006-267x.2016.05.017

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 5: 1412 - 1424

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.05.017

AQUACULTURE NUTRITION

Dietary Arginine Requirement of Genetically Improved Farmed Tilapia (Oreochromis niloticus)

WU Wenyi ,
JIANG Ming ,
LIU Wei ,
WU Fan ,
TIAN Juan ,
YANG Changgeng ,
WEN Hua

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1. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;
2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization of Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan 430223, China

Received date: 2015-11-17

Online published: 2016-05-24

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Abstract

This experiment was conducted to study the effects of dietary arginine (Arg) level on growth performance, feed utilization, body composition, serum biochemical indices and non-specific immune indices of genetically improved farmed tilapia (GIFT, Oreochromis niloticus), in order to determine the dietary Arg requirement of GIFT. Six isonitrogenous and isoenergetic semipurified diets (contained 30.21% crude protein and 17.77 MJ/kg gross energy) were formulated with 0.65%, 0.94%, 1.24%, 1.58%, 1.86% and 2.11% Arg, respectively. Each experimental diet was fed to 3 net cages for 60 d, and 15 fish with the initial body weight of (81.52±2.00) g in each net cage. The results showed as follows:with the dietary Arg level increasing, the weight gain rate (WGR), specific growth rate (SGR), protein efficiency ratios (PER) and protein deposition efficiency (PDE) were firstly increased and then decreased, and reached their peaks in the 1.58% group, which were significantly higher than those in other groups (P<0.05); whereas the feed conversion ratio (FCR) was firstly decreased and then increased, and reached the lowest value in the 1.58% group, which was significantly lower than that in other groups (P<0.05); the hepatosomatic index (HSI) and viscerosomatic index (VSI) had the same trend with FCR. The change tendency of ash content in muscle and crude lipid content in whole body was similar to FCR with dietary Arg level increasing, but the change tendency of crude lipid content in muscle was similar to WGR and SGR, while the crude protein content in muscle and ash content in whole body were firstly increased with dietary Arg level increasing and then leveled off. Dietary Arg level had no significant effects on the condition factor (CF) and the contents of crude protein in whole body, moisture in whole body and muscle (P>0.05). With the increase of dietary Arg level, the contents of all amino acids (except tyrosine, glycine and alanine), essential amino acids and total amino acids in muscle were firstly decreased and then increased. Dietary Arg level significantly affected the activities of aspartate transaminase (AST), alanine aminotransferase (ALT) and the contents of total protein (TP), urea nitrogen (UN), total cholesterol (T-CHO), glucose (GLU) and triglycerides (TG) in serum (P<0.05), and significantly affected the activities of total superoxide dismutase (T-SOD), total nitric oxide synthase (T-NOS) and lysozyme (LYZ) in liver and serum (P<0.05). Regarding the WGR, FCR and PER as the evaluation indicators, the quadratic regression analysis shows that the dietary Arg requirement of GIFT is 1.51% to 1.58% of diet or 4.99% to 5.25% of dietary protein.

Key words： GIFT (Oreochromis niloticus); arginine; requirement; growth; immune

Cite this article

WU Wenyi , JIANG Ming , LIU Wei , WU Fan , TIAN Juan , YANG Changgeng , WEN Hua . Dietary Arginine Requirement of Genetically Improved Farmed Tilapia (Oreochromis niloticus)[J]. Chinese Journal of Animal Nutrition, 2016 , 28(5) : 1412 -1424 . DOI: 10.3969/j.issn.1006-267x.2016.05.017

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