SPECIAL ECONOMIC ANIMAL NUTRITION

Effects of Dietary Methionine Level on Growth Performance, Serum Immune Indices and Antioxidant Indices of 9 to 10-Month-Old Porcupine

  • WANG Qianguang ,
  • LIU Qiu ,
  • ZHANG Baoping ,
  • GAO Huilin ,
  • TONG Xueqin ,
  • REN Guoji
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  • 1. Changde Vocational Technical College, Changde 415000, China;
    2. Changde Animal Husbandry Bureau, Changde 415000, China;
    3. The Duijin Porcupine Breeding Base in Taoyuan County, Changde 415000, China

Received date: 2015-11-29

  Online published: 2016-05-24

Abstract

This study was conducted to investigate the effects of dietary methionine (Met) level on growth performance, serum immune indices and antioxidant indices of 9 to 10-month-old porcupine, and to determine the Met requirement of 9 to 10-month-old porcupine based on quadratic regression model. Ninety-six 9-month-old porcupines with the similar body weight were randomly assigned to 6 groups with 4 replicates per group and each replicate contained 4 individuals. Porcupines in 6 groups were fed experimental diets with 0.25%, 0.45%, 0.65%, 0.85%, 1.05% and 1.25% Met, respectively. The adaption period lasted for 7 days and the trail period lasted for 50 days. The results showed as follows:1) dietary Met level did not significantly affect average daily gain (ADG) and average daily feed intake (ADFI) (P>0.05) of porcupine, but significantly affected feed/gain (F/G) (P<0.05). With the increase of dietary Met level, the F/G showed the tendency of a quadratic curve dropped firstly and then increased, and it had the minimum value of 3.63 when the Met level was 0.85%. 2) Dietary Met level had no significant effects on the contents of immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM) and the activity of superoxide dismutase (SOD) in serum (P>0.05), but significantly affected the activity of glutathione peroxidase (GSH-Px) (P<0.05) and the content of malonaldehyde (MDA) in serum (P<0.01). With the increase of dietary Met level, the activity of serum GSH-Px showed the tendency of a quadratic curve increased firstly and then decreased, and it had the maximum value when the Met level was 0.85%. The serum MDA content showed the tendency of a quadratic curve dropped firstly and then increased with the increase of dietary Met level. The content of serum MDA in the groups with 0.85% and 1.05% Met were significantly lower than those in the groups with 0.25%, 0.45% and 0.65% Met (P<0.01). The content of serum MDA in the group with 1.25% Met was significantly lower than that in the groups with 0.25% and 0.45% Met (P<0.01). In conclusion, dietary supplemented with proper Met can improve the growth performance and enhance the antioxidant ability of 9 to 10-month-old porcupine. The estimated Met requirements of 9 to 10-month-old porcupine to gain the minimum of F/G, the maximum of serum GSH-Px activity and the minimum of serum MDA content are 0.76%, 0.87% and 0.96% base on quadratic regression model, respectively.

Cite this article

WANG Qianguang , LIU Qiu , ZHANG Baoping , GAO Huilin , TONG Xueqin , REN Guoji . Effects of Dietary Methionine Level on Growth Performance, Serum Immune Indices and Antioxidant Indices of 9 to 10-Month-Old Porcupine[J]. Chinese Journal of Animal Nutrition, 2016 , 28(5) : 1425 -1432 . DOI: 10.3969/j.issn.1006-267x.2016.05.018

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