FEED SCIENCE AND TECHNOLOGY

Effects of Probiotics to Against Acute Diarrhea and on Intestinal Mucosal Barrier of Rats

  • PANG Min ,
  • LU Qingping ,
  • ZHU Liyuan ,
  • XIA Bing ,
  • ZHANG Hongfu
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  • State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China

Received date: 2015-12-07

  Online published: 2016-05-24

Abstract

The purpose of this study was to evaluate the effects of Clostridium butyricum or Lactobacillus salivarius ZJ614 on prevention of acute diarrhea and protection of intestinal mucosal barrier function. Forty-eight 21-day-weanned Wistar rats with the average body weight of (57.45±6.23) g were selected and randomly divided into four groups:normal control group (C1 group), model control group (C2 group), Clostridium butyricum group (T1 group) and Lactobacillus salivarius ZJ614 group (T2 group). Each group had six replicates, and each replicate had two rats. This experiment lasted for 9 days. C1 and C2 groups were orally administered saline, and T1 and T2 groups were administered probiotics accordingly. On the 8th day, every group except C1 group was administered folium senna to cause acute diarrhea and then record diarrhea condition of rats. All rats were sacrificed, and blood and intestinal samples were collected at the end of experiment. The results showed as follows:1) all rats displayed diarrhea symptoms as long as administered folium senna. Loose stools rate, diarrhea index, serum endotoxin and D-lactic acid contents were significantly increased in C2 group compared with C1 group (P<0.01). The diarrhea index, serum endotoxin and D-lactic acid contents decreased significantly in T1 and T2 groups compared with C2 group (P<0.01). 2) Compared with C2 group, the crypt depth in T1 and T2 groups was significantly decreased (P<0.05), the ratio of villus height to crypt depth in T1 and T2 groups was significantly increased (P<0.01), and the relative expression levels of ileum zonula occludens-1(ZO-1) (P<0.01) and occludin mRNA (P<0.05) in T1 group were significantly improved. 3) C2 group significantly up-regulated the relative expression levels of ileum interleukin-10(IL-10) (P<0.01)and interleukin-1β (IL-1β) mRNA (P<0.05) compared with C1 group, and T1 group down-regulated the relative expression level of IL-10 mRNA compared with C2 group (P<0.01). In summary, Clostridium butyricum or Lactobacillus salivarius ZJ614 can alleviate the actue diarrhea induced by senna on a certain degree, and also decrease the intestinal permeability, and enhance intestinal mucosal barrier.

Cite this article

PANG Min , LU Qingping , ZHU Liyuan , XIA Bing , ZHANG Hongfu . Effects of Probiotics to Against Acute Diarrhea and on Intestinal Mucosal Barrier of Rats[J]. Chinese Journal of Animal Nutrition, 2016 , 28(5) : 1462 -1470 . DOI: 10.3969/j.issn.1006-267x.2016.05.022

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