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Effects of Fusarium Toxins on Vulva, Reproductive Organ Index, Distribution and Expression of Estrogen Receptors in Uterus of Weaning Gilts

  • NIU Qunsheng ,
  • YANG Weiren ,
  • HUANG Libo ,
  • ZHANG Chongyu ,
  • ZHANG Guiguo ,
  • LIANG Caizhi ,
  • JIANG Shuzhen
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  • College of Animal Science and Technology, Shandong Agricultural University, Taian 271018, China

Received date: 2015-12-04

  Online published: 2016-05-24

Abstract

The aims of the study were to investigate the effect of Fusarium toxins on vulva, reproductive organ index, distribution and expression of estrogen receptors (ERs) of uterus in weaning gilts. A total of 40 healthy weaning gilts (Duroc×Large White×Landrace) aged at 35 d with an average body weight of (8.45±0.94) kg were used in the study. Gilts were randomly allocated into 2 treatments with 20 gilts in each group. Control group was fed a basal diet, and experimental group were exposed to feed naturally contaminated with Fusarium toxins[zearalenone (ZEN) 0.90 mg/kg; deoxynivalenol (DON) 1.43 mg/kg; fumonisin (FUM) 5.85 mg/kg] for 35 days after 7 days adaptation. The results showed that the length, width and area of vulva and reproductive organ index of weaning gilts at 35 days in experimental group were significantly higher than those in control group (P<0.05). There was a higher intensity of immunopositive staining for estrogen receptors α (ERα) in cytoplasm of endometrial glandular epithelial cells and uterine muscle cells. Whereas immunopositive staining for estrogen receptors β (ERβ) was most expressed in cytoplasm of smooth muscle cells in the intima of arteries and muscle layer smooth muscle cells. The ERα positive reaction and acinus number of the endometrial glandular epithelial cells in experimental groups were higher than that in control group. However, the ERβ positive reaction of the toxins-treated gilts was found in the smooth muscle cells of the uterine muscle and the arterial wall smooth muscle cells. The relative mRNA expressions of ERα and ERβ in uterus of weaning gilts in experimental group were significantly higher than those in control group (P<0.05). The results suggested Fusarium toxins have deleterious effect on reproductive system. This effect is mediated through changing the expression of ERs regulated by the transcription of ERs gene in uterus, and then changed the development of the reproductive organs.

Cite this article

NIU Qunsheng , YANG Weiren , HUANG Libo , ZHANG Chongyu , ZHANG Guiguo , LIANG Caizhi , JIANG Shuzhen . Effects of Fusarium Toxins on Vulva, Reproductive Organ Index, Distribution and Expression of Estrogen Receptors in Uterus of Weaning Gilts[J]. Chinese Journal of Animal Nutrition, 2016 , 28(5) : 1525 -1533 . DOI: 10.3969/j.issn.1006-267x.2016.05.029

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