Effects and Gene Regulatory Mechanism of Polysavone on Cholesterol Metabolism of Laying Hens

LI Ning; LIU Xin; QU Zhengxiang; LIU Tong; DU Yu; GAO Yupeng; MIN Yuna

doi:10.3969/j.issn.1006-267x.2016.05.033

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 5: 1558 - 1565

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.05.033

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Effects and Gene Regulatory Mechanism of Polysavone on Cholesterol Metabolism of Laying Hens

LI Ning ,
LIU Xin ,
QU Zhengxiang ,
LIU Tong ,
DU Yu ,
GAO Yupeng ,
MIN Yuna

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College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China

Received date: 2015-11-19

Online published: 2016-05-24

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Abstract

The experiment was carried out to evaluate the effects of dietary different polysavone supplemental level on cholesterol metabolism of laying hens, and discussed its regulation mechanism of gene expression. A total of 540 twenty-six-week-old Nick laying hens were randomly divided into 5 groups with 6 replicates per group and 18 birds per replicate. Laying hens in control group were fed with a basal diet, and other were fed with the basal diets supplemented with 300, 600, 900 and 1200 mg/kg polysavone, respectively. The pre-test lasted for 7 days, and the experiment lasted for 70 days. The results showed as follows:1) on day 35 and day 70, the yolk cholesterol content in 900 mg/kg polysavone group was significantly lower than that in control group (P<0.05). 2) Compared with the control group, the high density lipoprotein-cholesterol (HDL-C) content and high density lipoprotein to low density lipoprotein ratio (HDL/LDL) in 900 mg/kg polysavone group significantly increased (P<0.05). There were no significant differences in the contents of total cholesterol (TC), triglyceride (TG) and low density lipoprotein-cholesterol (LDL-C) in serum among all groups (P>0.05). 3) Compared with the control group, the mRNA expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) in liver of laying hens in 600, 900 and 1200 mg/kg polysavone groups significantly decreased (P<0.05), and the mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) in liver of laying hens in 600 and 900 mg/kg polysavone groups significantly increased (P<0.05). There were no significant differences in the mRNA expression of sterol regulatory element-binding protein 2(SREBP-2) in liver and occyte vitellogenesis receptor (OVR) in ovarian of laying hens among all groups (P>0.05). Our present study indicates that dietary supplemented with polysavone can reduce the whole egg and egg yolk cholesterol content. The possible mechanism of cholesterol lowering effects can result from reducing endogenous synthesis and more cholesterol excretion. The suitable dietary polysavone supplemented level of laying hens aged from 26 to 35 weeks is 900 mg/kg.

Key words： polysavone; feeding; laying hens; cholesterol metabolism; gene expression

Cite this article

LI Ning , LIU Xin , QU Zhengxiang , LIU Tong , DU Yu , GAO Yupeng , MIN Yuna . Effects and Gene Regulatory Mechanism of Polysavone on Cholesterol Metabolism of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2016 , 28(5) : 1558 -1565 . DOI: 10.3969/j.issn.1006-267x.2016.05.033

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