This research was carried out to investigate the effects of Bacillus megaterium 1259(BM1259) contrast with Yucca and Bacillus subtillis on performance and the content of ammonia nitrogen in excrement of laying hens. A total of three hundred and eighty four 300-day-old healthy Hy-line variety brown laying hens with similar laying rate were selected and randomly assigned into four groups with six replicates per group and sixteen hens per replicate. Birds in the control group were fed a basal diet, and the others in experimental groups were fed the basal diet supplemented with 100 mg/kg BM1259, Yucca and Bacillus subtillis, respectively. The preliminary period lasted for 14 days and the experiment lasted for 42 days. The results showed as follows:1) compared with the control group, the total egg mass, egg production, laying rate and average egg weight in BM1259 group were significantly increased (P<0.01), and the feed to egg ratio was significantly decreased (P<0.01), which was similar to Bacillus subtillis group, better than Yucca group. 2) Compared with the control group, the ammonia nitrogen, usea nitrogen and uric acid contents and uricase activity in BM1259 group were significantly decreased (P<0.01), which was similar to Yucca group, better than Bacillus subtillis group. 3) Dietary supplanted with BM1259 had more profit than Yucca and Bacillus subtillis in this study. In conclusion, dietary supplemented with BM1259 can improve performance, increase profit and better remove ammonia nitrogen and odor in the excrement of laying hens.
DING Wenjun
,
WANG Qiang
,
DAI Meimei
,
ZHANG Weili
,
ZHANG Yanyun
. Effects of Bacillus megaterium 1259 Contrast with Yucca and Bacillus subtillis on Performance and Ammonia Nitrogen Content in Excrement of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2016
, 28(5)
: 1566
-1572
.
DOI: 10.3969/j.issn.1006-267x.2016.05.034
[1] WATHES C M,JONES J B,KRISTENSEN H H,et al.Aversion of pigs and domestic fowl to atmospheric ammonia[J].Transactions of the ASAE,2002,45(5):1605-1610.
[2] 中华人民共和国农业部.《饲料添加剂品种目录(2013)》[中华人民共和国农业部公告 第2045号] [EB/OL].[2014-01-03].http://www.hytwp99.com/content/?400.html.
[3] 苏子峰,张红兵,樊月圆,等.丝兰提取物与枯草芽孢杆菌对肉鸡生长性能及鸡舍有害气体的影响[J].饲料博览,2012(6):1-5.
[4] 王晓霞,易中华,计成,等.果寡糖和枯草芽孢杆菌对肉鸡肠道菌群数量、发酵粪中氨气和硫化氢散发量及营养素利用率的影响[J].畜牧兽医学报,2006,37(4):337-341.
[5] 张艳云,霍永久,戴承墉.巨大芽孢杆菌制剂对蛋鸡舍氨和硫化氢产生量的影响[C]//第二届全国畜禽和水产养殖污染监测与控制治理技术交流研讨会.厦门:中国环境科学学会,2008.
[6] 中华人民共和国农业部.中华人民共和国农业行业标准 家禽生产性能名词术语和度量统计方法[J].中国禽业导刊,2006,23(15):45-46.
[7] 蒲万霞,魏云霞,孟晓琴,等.靛酚蓝-分光光度法测定胃肠道内容物中氨态氮含量研究[J].甘肃农业大学学报,2008,43(5):13-17.
[8] 关松荫.土壤酶及其研究方法[M].北京:农业出版社,1986:295-297.
[9] 丁文骏,张艳云,杨海明,等.芽孢杆菌制剂在鸡生产中的研究与应用[J].饲料研究,2015(13):22-25,30.
[10] 龚红,邹胜龙.丝兰提取物的研究与应用[J].饲料研究,2015(1):14-17,39.
[11] ABDELQADER A,AL-FATAFTAH A R,DA Ş G.Effects of dietary Bacillus Subtilis and inulin supplementation on performance,eggshell quality,intestinal morphology and microflora composition of laying hens in the late phase of production[J].Animal Feed Science and Technology,2013,179(1/2/3/4):103-111.
[12] MOLNÁR A K,PODMANICZKY B,KVRTI P,et al.Effect of different concentrations of Bacillus Subtilis on growth performance,carcase quality,gut microflora and immune response of broiler chickens[J].British Poultry Science,2011,52 (6):658-665.
[13] 王建彬,刘晋娟,李小红,等.枯草杆菌培养物降低养鸡场氨气排放的研究报告[J].当代畜牧,2008(11):50-52.
[14] 程志斌,樊月圆,张红兵,等.丝兰提取物与枯草芽孢杆菌对肉鸡舍臭气影响[J].饲料研究,2012(7):25-27.
[15] 马彦博,白东英,朱松波,等.丝兰属植物提取物降低鸡舍氨气浓度的研究[J].畜禽业,2003(10):14-15.
[16] 贾淑庚,任灵肖,梁剑峰,等.丝兰属植物提取物对蛋鸡生产性能及蛋品质的影响[J].中国家禽,2014,36(16):34-37.
[17] 张为利,喻礼怀,肖天荣,等.BM1259与丝兰素对蛋鸡排泄物存放过程中氨氮释放作用的比较研究[J].饲料工业,2014,35(16):13-16.
[18] 丁文骏,王强,戴美梅,等.巨大芽孢杆菌1259对产蛋鸡生产性能及排泄物中含氮物浓度的影响[J].动物营养学报,2015,27(10):3140-3145.