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Effects of Dried Oat Hay Content in Diet on Rumen Fluid pH and Microflora of Sheep

  • ZHOU Rui ,
  • ZHAO Shengguo ,
  • LIU Lishan ,
  • WANG Chuan ,
  • WU Jianping
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  • 1. College of Animal Science & Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2015-11-23

  Online published: 2016-05-24

Abstract

In order to investigate the effects of dried oat hay content in diet on rumen fluid pH and microflora of sheep. Nine German merino sheep×Ongolian sheep cross breed rams with similar body condition and body weight[(70.32±2.14) kg] were used in a replicated 3×3 Latin square design, and randomly divided into 3 groups with 3 rams per group. Sheep were fed whole corn silage, whole corn silage+dried oat hay (1:1) (mixed group) and dried oat hay as roughages, and the dietary concentrate to roughage ratio was 34.50:65.50. The experimental consisted of 3 periods with 20 d per period, and each period had 15 d for pre-test and 5 d for sampling. Rumen fluid was collected before feeding (0 h) and 1, 3, 5 and 7 h after feeding to measure pH, meanwhile, the relative contents of microorganisms with real-time quantitative PCR method. The results showed as follows:1) ruminal pH of whole corn silage group was significantly lower than that of dried oat hay group at 1 and 5 h (P<0.05), extremely significantly lower than that of mixed group at 3 h (P<0.01); 2) the relative content of fungus in rumen fluid of mixed group and dried oat hay group was extremely significantly greater than that of whole corn silage at 0 h (P<0.01), and dried oat hay group was also significantly higher than whole corn silage group (P<0.05); 3) the relative content of protozoa in rumen fluid of mixed group was significantly lower than that of whole corn silage group at 1 and 5 h (P<0.05); 4) after 5 h of feeding, the relative contents of cellulolytic bacteria of mixed group and dried oat hay group were relatively high, among which the relative content of Ruminococcus flavefaciens was significantly higher than that of whole corn silage group (P<0.05), the relative contents of Ruminococcus albus and Fibrobacter succinogenes were extremely significantly higher than those of whole corn silage group (P<0.01). In conclusion, it is benefit for maintain stable ecosystem of rumen and growth of microorganisms that using whole corn silage+dried oat hay (1:1) as roughage in diet with concentrate to roughage ratio of 34.50:65.50, and Ruminococcus albus and Fibrobacter succinogenes are dominant bacteria.

Cite this article

ZHOU Rui , ZHAO Shengguo , LIU Lishan , WANG Chuan , WU Jianping . Effects of Dried Oat Hay Content in Diet on Rumen Fluid pH and Microflora of Sheep[J]. Chinese Journal of Animal Nutrition, 2016 , 28(5) : 1589 -1597 . DOI: 10.3969/j.issn.1006-267x.2016.05.037

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