Effects of Dietary Cation-Anion Difference Level on Plasma Calcium Concentration and Gastrointestinal Tract Tissues Calcium Binding Proteins-D9k mRNA Relative Expression Level of Mice during the Transition Period

WU Wenxuan; WU Jiahai; ZHANG Jikun; XIN Hailiang; YANG Yi; ZHU Lunqin

doi:10.3969/j.issn.1006-267x.2016.06.013

2016 , Vol. 28 >Issue 6: 1726 - 1734

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.06.013

MOLECULAR NUTRITION

Effects of Dietary Cation-Anion Difference Level on Plasma Calcium Concentration and Gastrointestinal Tract Tissues Calcium Binding Proteins-D9k mRNA Relative Expression Level of Mice during the Transition Period

WU Wenxuan ,
WU Jiahai ,
ZHANG Jikun ,
XIN Hailiang ,
YANG Yi ,
ZHU Lunqin

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1. College of Animal Science, Institute of New Rural Development, Guizhou University, Guiyang 550025, China;
2. Guizhou Institute of Prataculture, Guiyang 550006, China;
3. Institute of Animal Science, Jiangxi Academy of Agricultrual Sciences, Nanchang 330200, China;
4. Qiandongnan National Polytechnic, Kaili 556000, China

Received date: 2015-12-28

Online published: 2016-06-27

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Abstract

Based on the calcium related gene expression profile and its analysis, the study was conducted to investigate the effects of dietary cation-anion difference (DCAD) level on plasma calcium concentration and gastrointestinal tract tissues calcium binding proteins-D9k (CaBP-D9k) relative expression level of mice during the transition period, and to clarify the mechanism of low DCAD level prevent hypocalcaemia. One hundred and twenty KM mice were randomly allotted into 3 groups with 40 mice in each group, mice in the 3 groups were fed 3 diets with DCAD level at +300 (high DCAD level group, HD group), +150 (control group, CON group), and -150 (low DCAD level group, LD group), respectively. Plasma calcium concentration and gastrointestinal tract tissues CaBP-D9k mRNA relative expression level were detected at 4 time points: day of 20 (-20 d) and 5 (-5 d) before kidding, day of kidding (0 d), and day of 3 after kidding (+3 d). The results showed that compared with the HD group, the plasma calcium concentration of mice at 0 d and +3 d during the transition period in LD group was significantly increased (P<0.05), and the CaBP-D9k mRNA relative expression level was significantly up-regulated in duodenum, jejunum, ileum and colon (P<0.05), especially observed on -5 d before parturition and 0 d at parturition (P<0.05). Also, CaBP-D9k mRNA relative expression level was significantly increased in jejunum and colon on the +3 d after parturition (P<0.05). Moreover, there was a significant correlation between CaBP-D9k mRNA relative expression level in duodenum, jejunum and colon and plasma calcium concentration. In conclusion, low DCAD level is able to up-regulate CaBP-D9k mRNA relative expression level in small intestine and colon, accompany with greater plasma calcium concentration. This is possibly an important way of low DCAD level maintains plasma calcium homeostasis during the transition period, and reduce the occurrence rate of hypocalcemia.

Key words： dietary cation-anion difference; plasma calcium concentration; calcium-binding protein; gastrointestinal tract tissues

Cite this article

WU Wenxuan , WU Jiahai , ZHANG Jikun , XIN Hailiang , YANG Yi , ZHU Lunqin . Effects of Dietary Cation-Anion Difference Level on Plasma Calcium Concentration and Gastrointestinal Tract Tissues Calcium Binding Proteins-D9k mRNA Relative Expression Level of Mice during the Transition Period[J]. Chinese Journal of Animal Nutrition, 2016 , 28(6) : 1726 -1734 . DOI: 10.3969/j.issn.1006-267x.2016.06.013

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