Study on Intestinal Damage Repair of Epidermal Growth Factor on Dextra Sulfate Sodium-Induced Colitis in Mice

LIU Shujie; DENG Bo; XU Ziwei

doi:10.3969/j.issn.1006-267x.2016.06.014

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 6: 1735 - 1741

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.06.014

FEED SCIENCE AND TECHNOLOGY

Study on Intestinal Damage Repair of Epidermal Growth Factor on Dextra Sulfate Sodium-Induced Colitis in Mice

LIU Shujie ,
DENG Bo ,
XU Ziwei

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Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received date: 2016-03-09

Online published: 2016-06-27

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Abstract

This study aimed to evaluate the effects of intestinal damage repair of epidermal growth factor (EGF) on dextra sulfate sodium (DSS)-induced colitis in mice. Twenty-four BALB/C mice aged at 6 weeks were randomly assigned into three groups: normal control group, DSS model control group and DSS+EGF group. The normal control group received normal drinking water; DSS model control group received 5% DSS solution in drinking water for 7 consecutive days and followed with normal drinking water for 3 days; DSS+EGF group received the same treatment of DSS as DSS model control group, meanwhile, these mice were administered with EGF by intraperitoneal injection twice a day and for 10 days. The results showed as follows: 1) DSS model control group significantly shortened colon length of mice compared with the normal control group (P<0.01), and DSS+EGF group significantly increased colon length of mice compared with the DSS model control group (P<0.01). 2) Mice in DSS model control group revealed a typical ulcer of the colonic tissues, and significant increased colonic damage score (CDS) compared with the normal control group (P<0.01). Mice in DSS+EGF group did not show the ulcer of the colonic tissues, and significantly reduced CDS compared with DSS model control group (P<0.01). 3) DSS model control group significantly decreased colonic tight junction proteins (Occludin) concentration of mice compared with normal control group (P<0.05). DSS+EGF group significantly increased colonic Occludin concentration of mice compared with DSS model control group (P<0.05). 4) DSS model control group significantly decreased colonic interleukin (IL)-2 (P<0.01), IL-4 (P<0.01) and IL-10 concentrations (P<0.05) of mice compared with normal control group. DSS+EGF group significantly increased colonic IL-2 (P<0.01), IL-4 (P<0.01) and IL-10 concentrations (P<0.05) of mice compared with DSS model control group.The results show that EGF can repair damaged intestinal tissue and maintain the integrity of the intestinal mucosal barrier by increasing intestinal Occludin expression and regulation of levels of intestinal cytokines concentrations.

Key words： epidermal growth factors; intestinal tract; damage repair; colitis

Cite this article

LIU Shujie , DENG Bo , XU Ziwei . Study on Intestinal Damage Repair of Epidermal Growth Factor on Dextra Sulfate Sodium-Induced Colitis in Mice[J]. Chinese Journal of Animal Nutrition, 2016 , 28(6) : 1735 -1741 . DOI: 10.3969/j.issn.1006-267x.2016.06.014

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