Effects of Bacillus subtilis on Growth Performance, Intestinal Morphology and Bacterial Enumeration of Broilers

QI Bo; WU Shugeng; WANG Jing; QI Guanghai; ZHANG Haijun; YAN Guiling; WANG Xu; DONG Yan

doi:10.3969/j.issn.1006-267x.2016.06.016

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 6: 1748 - 1756

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.06.016

FEED SCIENCE AND TECHNOLOGY

Effects of Bacillus subtilis on Growth Performance, Intestinal Morphology and Bacterial Enumeration of Broilers

QI Bo ,
WU Shugeng ,
WANG Jing ,
QI Guanghai ,
ZHANG Haijun ,
YAN Guiling ,
WANG Xu ,
DONG Yan

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1. Feed Research Institute, Chinese Academy of Agricultural Sciences, Laboratory of Feed Biotechnology of Ministry of Agriculture, National Engineering Research Center of Biological Feed, Beijing 100081, China;
2. Evonik Degussa(China) Co., Ltd., Beijing 100026, China

Received date: 2015-12-17

Online published: 2016-06-27

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Abstract

This study was conducted to investigate the effects of Bacillus subtilis on growth performance, slaughter performance, intestinal morphology and bacterial enumeration of broilers. A total of 320 one-day-old healthy male Arbor Acres broilers with similar weight were randomly allotted into 4 groups with 8 replicates per group and 10 broilers per replicate. Chickens in control group were fed a basal diet, and those in experimental groups were fed basal diets supplemented with 500 mg/kg Bacillus subtilis (BS group), 20 mg/kg colistin sulfate (CS group), and 500 mg/kg Bacillus subtilis +10 mg/kg colistin sulfate (BS+CS group), respectively. The test lasted for 6 weeks. The results showed as follows: 1) compared with control group, average body weight of broilers in BS and CS groups was significantly increased at 42 days of age (P<0.05), average daily gain of broilers in BS and CS groups was increased during 22 to 42 days of age, and the ratio of feed to gain of broilers in BS and CS groups was significantly decreased during 22 to 42 days of age and 1 to 42 days of age (P<0.05). The growth performance of broilers in BS+CS group had no significantly changes during every experimental period (P>0.05). 2) There was no significant difference in slaughter performance of broilers among all groups (P>0.05). 3) Compared with control group, the villus height and the ratio of villus height to crypt depth in duodenum of broilers in BS group were significantly increased (P<0.05). 4) Compared with control group, Lactobacillus enumeration in cecum of broilers in BS, CS and BS+CS groups was significantly increased at 42 days of age (P<0.05).The ratio of Lactobacillus to Escherichia coli in cecum of broilers in BS and BS+CS groups was significantly increased at 42 days of age (P<0.05). Therefore, Bacillus subtilis can improve the intestinal morphology, increase Lactobacillus enumeration in cecum and promote the growth performance of broilers. In conclusion, the effect of Bacillus subtilis is better than that of colistin sulfate, but the effect of combination of Bacillus subtilis and colistin sulfate is not good.

Key words： Bacillus subtilis; colistin sulfate; growth performance; intestinal morphology; bacterial communities

Cite this article

QI Bo , WU Shugeng , WANG Jing , QI Guanghai , ZHANG Haijun , YAN Guiling , WANG Xu , DONG Yan . Effects of Bacillus subtilis on Growth Performance, Intestinal Morphology and Bacterial Enumeration of Broilers[J]. Chinese Journal of Animal Nutrition, 2016 , 28(6) : 1748 -1756 . DOI: 10.3969/j.issn.1006-267x.2016.06.016

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