Effects of Dietary Cholesterol Content on Growth Performance, Tissue Biochemical Indices and Liver Lipid Metabolism Related Enzyme Activities of Orange-Spotted Grouper (Epinephelus coioides)

ZHANG Wucai; DONG Xiaohui; TAN Beiping; ZHANG Shuang; CHI Shuyan; YANG Qihui; LIU Hongyu; CHEN Xiaoru

doi:10.3969/j.issn.1006-267x.2016.06.038

2016 , Vol. 28 >Issue 6: 1945 - 1955

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.06.038

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Effects of Dietary Cholesterol Content on Growth Performance, Tissue Biochemical Indices and Liver Lipid Metabolism Related Enzyme Activities of Orange-Spotted Grouper (Epinephelus coioides)

ZHANG Wucai ,
DONG Xiaohui ,
TAN Beiping ,
ZHANG Shuang ,
CHI Shuyan ,
YANG Qihui ,
LIU Hongyu ,
CHEN Xiaoru

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1. Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China;
2. Tongwei Co., Ltd., Chengdu 610041, China

Received date: 2015-12-23

Online published: 2016-06-27

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Abstract

A feeding trial was conducted to evaluate the effects of dietary cholesterol content on growth performance, tissue biochemical indices and liver lipid metabolism related enzyme activities of orange-spotted grouper (Epinephelus coioides). Taking casein as main protein source, six isoenergetic and isonitrogenous diets were formulated with the cholesterol adding levels of 0 (control group), 5, 10, 15, 20 and 25 g/kg, named as C0,C0.5,C1,C1.5,C2 and C2.5, and the measured values of cholesterol content in those diets were 2.6, 7.5, 12.7, 17.2, 22.2 and 27.2 g/kg, respectively, and then fed to 360 orange-spotted grouper with initial average body weight of (84.30±0.25) g for 10 weeks. Each diet was randomly assigned to three replicates with 20 fishes in each replicate. The results showed as follows: weight gain rate (WGR), protein efficiency ratio (PER) and feed efficiency (FE) were firstly increased and then decreased with the dietary cholesterol content increasing. The WGR, PER and FE of the C0.5 group were the highest and significantly higher than those of the control group (P<0.05). The whole body crude lipid content of adding groups was significantly higher than that of the control group (P<0.05), whereas the ash content of supplementation groups was significantly lower than that of the control group (P<0.05). The total cholesterol (TC) and triglyceride (TG) contents in liver were increased with the dietary cholesterol content increasing, but the muscle TC and TG contents were not affected by dietary cholesterol content (P>0.05). With dietary cholesterol content increasing, serum TC, TG, high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) contents were firstly increased and changed to be steady, and the HDL-C/LDL-C had a down trend. The serum TG content of the C2.0 group was significantly higher than that of the control group (P<0.05), the serum HDL-C content of the C2.5 group was significantly higher than that of the C0.5 and C1.5 groups, control group (P<0.05), the serum LDL-C content of the C2.5 group was significantly higher than that of the other groups (P<0.05), and the HDL-C/LDL-C of control group was significantly higher than that of the adding groups (P<0.05). With the dietary cholesterol content increasing, the activity of malic enzyme (ME) in liver was increased, and that of C1.5, C2.0 and C2.5 group was significantly higher than that of the other groups (P<0.05); the activity of carnitine acyltransferase Ⅰ(CPTⅠ) in liver had a downtrend and then to be stable, and that of control group was significantly higher than that of the other groups (P<0.05); the activity of cholesterol 7α hydroxylase (CYP7A1) had a uptrend and then to be stable, and that of the C0.5 group was the highest and increased by 35.48% than that of the control group (P<0.05). In this experiment, base on the WGR and liver CYP7A1 activity as evaluation indices, the cholesterol requirement for the orange-spotted grouper determined by broken-line model analysis is 7.43 and 8.70 g/kg diet, respectively.

Key words： orange-spotted grouper; cholesterol; growth performance; biochemical indices; lipid metabolism

Cite this article

ZHANG Wucai , DONG Xiaohui , TAN Beiping , ZHANG Shuang , CHI Shuyan , YANG Qihui , LIU Hongyu , CHEN Xiaoru . Effects of Dietary Cholesterol Content on Growth Performance, Tissue Biochemical Indices and Liver Lipid Metabolism Related Enzyme Activities of Orange-Spotted Grouper (Epinephelus coioides)[J]. Chinese Journal of Animal Nutrition, 2016 , 28(6) : 1945 -1955 . DOI: 10.3969/j.issn.1006-267x.2016.06.038

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