Effects of Egg Yolk Powder Including Vanadium on Growth, Oxidative Stress Status and Its Related Gene Expression of Wistar Rats

CUI Renyong; WANG Jianping; ZHANG Keying; DING Xuemei; ZENG Qiufeng; BAI Shiping; LUO Yuheng

doi:10.3969/j.issn.1006-267x.2016.06.039

2016 , Vol. 28 >Issue 6: 1956 - 1964

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.06.039

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Effects of Egg Yolk Powder Including Vanadium on Growth, Oxidative Stress Status and Its Related Gene Expression of Wistar Rats

CUI Renyong ,
WANG Jianping ,
ZHANG Keying ,
DING Xuemei ,
ZENG Qiufeng ,
BAI Shiping ,
LUO Yuheng

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Key Laboratory of Animal Disease-Resistance of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China

Received date: 2016-01-07

Online published: 2016-06-27

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Abstract

As a kind of heavy metal, excessive intake of vanadium can cause oxidative stress to laying hens, reduce egg quality and make residue in eggs, which affects the safety of eggs. The objective of this study was to explore the effects of feeding egg yolk powder including vanadium to Wistar rats on their growth, oxidative stress status and its related gene expression, and to evaluate the biosecurity of organic vanadium. A total of 27 female Wistar rats (4 weeks old) were allocated to 3 treatments with 9 rats each, and fed three kinds of diets supplemented with 600 g/kg egg yolk powders which were prepared with eggs from laying hens fed diets containing 0, 5 and 10 mg/kg vanadium, respectively. The three diets actually contained 0.107, 0.137 and 0.164 mg/kg vanadium. The experiment lasted for 35 d. The results showed that there were no significant differences on growth performance, organ indices, contents of plasma triglyceride, malonaldehyde (MDA) and urea nitrogen, activities of plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST), liver and kidney superoxide dismutase (SOD) activity, MDA content, and total antioxidant capacity (T-AOC), activities of liver ALT and AST, liver glutathione content, and liver glutathione S-transferase activity, as well as histomorphology of liver and kidney and vanadium residual in kidney of rats among 3 treatments (P>0.05). However, compared with treatment 1, liver quinone oxidoreductase 1 (NQO1) activity of rats in treatments 2 and 3 was significantly decreased (P<0.05), and the relative expression levels of liver NQO1 and nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA were significantly decreased (P<0.05), too. The results indicate that the dietary supplementation of 600 mg/kg egg yolk powders with different vanadium content has no significantly different effects on growth performance and oxidation-reduction state of Wistar rats. However, the NQO1 activity, mRNA expression of NQO1 and Nrf2 are down-regulated as the vanadium content increasing (0.137 and 0.164 mg/kg).

Key words： egg yolk powder; vanadium; rat; oxidative stress; NQO1; Nrf2

Cite this article

CUI Renyong , WANG Jianping , ZHANG Keying , DING Xuemei , ZENG Qiufeng , BAI Shiping , LUO Yuheng . Effects of Egg Yolk Powder Including Vanadium on Growth, Oxidative Stress Status and Its Related Gene Expression of Wistar Rats[J]. Chinese Journal of Animal Nutrition, 2016 , 28(6) : 1956 -1964 . DOI: 10.3969/j.issn.1006-267x.2016.06.039

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