Effects of Chitooligosaccharide on Lipopolysaccharide Induced Oxidative Damage in Epithelial Cells of Pig Jejunum

XIAO Dingfu; ZHONG Jia; LIU Jinhui; LI Wenping

doi:10.3969/j.issn.1006-267x.2016.07.013

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 7: 2090 - 2095

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.07.013

MOLECULAR NUTRITION

Effects of Chitooligosaccharide on Lipopolysaccharide Induced Oxidative Damage in Epithelial Cells of Pig Jejunum

XIAO Dingfu ,
ZHONG Jia ,
LIU Jinhui ,
LI Wenping

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1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China

Received date: 2016-01-07

Online published: 2016-07-28

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Abstract

This study was designed to investigate the effect of chitooligosaccharide (COS) on oxidative stress and used lipopolysaccharide (LPS) induced epithelial cells of pig jejunum (IPEC-J2) as an in vitro model of oxidative stress. The IPEC-J2 were incubated with LPS and COS for 12, 24 and 48 h,and then used methylthiazolyldiphenyl-tetrazolium bromide (MTT) method to detect the activity of cell proliferation; the appropriate concentrations of LPS (1.0 μg/mL) and COS (200 μg/mL) were chosen in the IPEC-J2 cells, divided into control group, LPS group, COS group and LPS+COS group, and then detected the expression of NF-E2-related factor 2 (Nrf2)and heme oxygenase 1 (HO-1) by Western Blot; and detected superoxide dismutase (SOD), catalase (CAT) activities and malonaldehyde (MDA) content by the kit. The results showed that the optimum concentration of LPS and incubated time for model was 1.0 μg/mL and 24 h, the inhibition of cell proliferation was 41.6%; COS promoted the proliferation of IPEC-J2, worked the best when its concentration was 200 μg/mL and incubated 24 h, the cell proliferation rate was 126.3%. Compared with the control group, LPS+COS group was not significantly different in SOD, CAT activities and MDA content (P>0.05), but Nrf2 and HO-1 protein expression was significantly increased (P<0.05); compared with LPS group, Nrf2 protein expression of LPS+COS group was significantly higher (P<0.05), and there was an increasing trend of HO-1 protein expression, but the difference was not significant (P>0.05), moreover, SOD, CAT activities were significantly increased and MDA content was significantly reduced (P<0.05). It is concluded that LPS induce oxidative stress of IPEC-J2, but COS can induce high expression of Nrf2 and HO-1 protein, and improve the activity of antioxidant enzymes, reduce the content of oxidative products, so increase cellular resistance to oxidative stress, and play a protective role.

Key words： chitooligosaccharide; lipopolysaccharide; IPEC-J2; oxidative stress; antioxidant

Cite this article

XIAO Dingfu , ZHONG Jia , LIU Jinhui , LI Wenping . Effects of Chitooligosaccharide on Lipopolysaccharide Induced Oxidative Damage in Epithelial Cells of Pig Jejunum[J]. Chinese Journal of Animal Nutrition, 2016 , 28(7) : 2090 -2095 . DOI: 10.3969/j.issn.1006-267x.2016.07.013

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