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Effects of Ruminal Acute Acidosis Induced by Fructo-Oligosaccharide on Ruminal Fermentation, Hooves Structure and Expressions of Inflammatory Cytokines and Matrix Metalloproteinase of Goats

  • FENG Panfei ,
  • LIU Junhua ,
  • YE Huimin ,
  • ZHU Weiyun ,
  • MAO Shengyong
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  • College of Animal Science, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2016-01-11

  Online published: 2016-07-28

Abstract

The present study aimed to investigate the effects of ruminal acute acidosis induced by fructo-oligosaccharide on ruminal fermentation, hooves structure and expressions of inflammatory cytokines and matrix metalloproteinase of goats. Eight healthy crossbred goats (Boer goat×Yangtze River delta white goats) with permanent ruminal fistulas were randomly divided into two groups, including control and test groups which was treated with fructo-oligosaccharide infusion (21 g/kg BW), and each group had 4 goats. Ruminal liquid and blood were collected respectively at 0 (before infusion), 4, 8, 12, 24 and 48 h, as well as 0, 4, 8, 24 and 48 h after infusion. The goats were slaughtered to collect for hooves tissue after 48 h of infusion. The results showed as follows, compared with control group, the average values of ruminal liquid pH, volatile fatty acids was significantly decreased in test group (P<0.05), while the average values of the concentrations of lactic acid and lipopolysaccharide (LPS) in ruminal liquid and blood were significantly increased (P<0.05); the results of histology suggested that compared with control group, secondary epidermal lamellae and secondary dermal lamellae were shortened in test group; the real-time PCR results revealed that compared with control group, the mRNA expression of tissue inhibitors of metalloproteinases-1 (TIMP-1) was significantly decreased in test group (P<0.05), while those of interleukin-6 (IL-6), matrix metalloproteinase 2 (MMP-2) and membrane type-1 matrix metalloproteinases (MT1-MMP) in goat hooves were significantly increased (P<0.05). In conclusion, ruminal acute acidosis induced by fructo-oligosaccharide causes the disorder of ruminal fermentation and increases concentrations of LPS and lactic acid in ruminal liquid and blood, which further changes of mRNA expressions of pro-inflammatory cytokines and matrix metalloproteinase genes related to laminitis, and finally induces the laminitis in goats.

Cite this article

FENG Panfei , LIU Junhua , YE Huimin , ZHU Weiyun , MAO Shengyong . Effects of Ruminal Acute Acidosis Induced by Fructo-Oligosaccharide on Ruminal Fermentation, Hooves Structure and Expressions of Inflammatory Cytokines and Matrix Metalloproteinase of Goats[J]. Chinese Journal of Animal Nutrition, 2016 , 28(7) : 2260 -2268 . DOI: 10.3969/j.issn.1006-267x.2016.07.033

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