Comparative Analysis: Nutritional Component Contents in Cuttlebone of Sepia pharaonis at Different Growth Stages

JIANG Maowang; JIANG Xiamin; LIANG Jingjing; WANG Pengshuai; RUAN Peng; HAN Qingxi

doi:10.3969/j.issn.1006-267x.2016.07.038

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 7: 2300 - 2308

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.07.038

SHORT COMMUNICATIONS

Comparative Analysis: Nutritional Component Contents in Cuttlebone of Sepia pharaonis at Different Growth Stages

JIANG Maowang ,
JIANG Xiamin ,
LIANG Jingjing ,
WANG Pengshuai ,
RUAN Peng ,
HAN Qingxi

Expand

School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2016-01-18

Online published: 2016-07-28

Fold

Abstract

This study was conducted to study the changes of nutritional component contents in cuttlebone of Sepia pharaonis at different growth stages, in order to provide a theoretical basis for development and utilization of Sepia pharaonis. The contents of nutritional components (including moisture, ash, crude protein, crude fat, polysaccharide, amino acids, fatty acids) and minerals [including 5 kinds of major elements-natrium (Na), kalium (K), magnesium (Mg), aluminium (Al), calcium (Ca), and 8 kinds of trace elements-copper (Cu), iron (Fe), zinc (Zn), selenium (Se), manganese (Mn), chromium (Cd), arsenic (As), strontium (Sr)] in cuttlebone of Sepia pharaonis at different growth stages (growing ages were 60, 90, 120 and 150 d, respectively) were determined using national standard nutritional methods. The results showed as follows: among different growth stages, the ash was the main component in cuttlebone of Sepia pharaonis and the content of ash was 89.98% to 92.06%, which was increased with the growth and 150 days of age was significantly higher than other days of age (P<0.05); crude protein content was 2.82% to 3.11%, which was increased with the growth and 150 days of age was significantly higher than 60 days of age (P<0.05); the content of moisture was 3.93% to 6.21%, which was decreased with the growth and 60 days of age was significantly higher than other days of age (P<0.05); the contents of crude fat and polysaccharide were less compared with other components, which were 0.41% to 0.47% and 0.34% to 0.38%, respectively. A total of 17 amino acids were identified in cuttlebone of Sepia pharaonis among different growth stages, including 7 kinds of essential amino acids (EAA). The contents of total amino acid (TAA), EAA and acidic amino acids (AAA) were 2.79% to 2.93%, 0.87% to 0.98% and 0.56% to 0.78%, respectively; the content of AAA was increased with the growth, and 150 days of age was significantly higher than other days of age (P<0.05). The content of glutamate (Glu) was the highest (0.31% to 0.43%) and the content of histidine (His) was the lowest (0.03% to 0.07%) in cuttlebone of Sepia pharaonis among different growth stages. A total of 7 fatty acids were identified, including 3 saturated fatty acids (SFA), 1 monounsaturated fatty acids (MUFA) and 3 polyunsaturated fatty acids (PUFA). C22: 6n-3, C16: 0 and C18: 0 were the main components of fatty acids, among which C22: 6n-3 had the highest value (35.68% to 37.62%). The contents of major elements-Na, Mg, Al and Ca, and trace elements-Cu, Zn and Sr were significantly affected by the growth stage (P<0.05), especially the contents of Ca, Mg and Zn, which were increased with the growth; the contents of heavy metal elements-Cd and As were very low, which were 0.001 to 0.003 mg/g and 0.012 to 0.030 mg/g, respectively. It is concluded that the cuttlebone of Sepia pharaonis has a high nutritional value, Ca, Mg and Zn contents are rich and increase with the growth. Therefore, it has a good spectacle of opening up and application.

Key words： Sepia pharaonis; cuttlebone; different growth stages; nutritional components; mineral elements

Cite this article

JIANG Maowang , JIANG Xiamin , LIANG Jingjing , WANG Pengshuai , RUAN Peng , HAN Qingxi . Comparative Analysis: Nutritional Component Contents in Cuttlebone of Sepia pharaonis at Different Growth Stages[J]. Chinese Journal of Animal Nutrition, 2016 , 28(7) : 2300 -2308 . DOI: 10.3969/j.issn.1006-267x.2016.07.038

References

[1] 高学敏.中药学[M].北京:中国中医药出版社,2002:582.
[2] 陶凯,毛天球,陈富林,等.一种新型羟基磷灰石材料的制备及其细胞相容性探讨[J].生物医学工程学杂志,2006,23(4):887-890.
[3] 周蔚,吴海涛,李采.海螵蛸作为组织工程支架材料的实验研究[J].复旦学报:医学版,2007,34(3):438-441.
[4] 刘源.海螵蛸/骨形态发生蛋白复合人工骨成骨及再血管化的初步研究[D].硕士学位论文.大连:大连医科大学,2012.
[5] AKSAKAL B,DEMIREL M.Synthesis and fabrication of novel cuttlefish (Sepia officinalis) backbone biografts for biomedical applications [J].Ceramics International,2015,41(3):4531-4537.

[6] CHUNG M T,WANG C H.Age validation of the growth lamellae in the cuttlebone from cultured Sepia pharaonis at different stages[J].Journal of Experimental Marine Biology and Ecology,2013,447:132-137.
[7] 唐丽娟,刘玮炜,王丽,等.不同方法提取海螵蛸多糖及结构表征[J].中成药,2011,33(9):1625-1628.
[8] 唐丽娟,刘玮炜,史大华,等.海螵蛸提取物对脲酶活性的抑制作用初探[J].中华中医药杂志,2012,27(2):331-333.
[9] RAMASAMY P,SUBHAPRADHA N,SHANMUGAM V,et al.Extraction,characterization and antioxidant property of chitosan from cuttlebone Sepia kobiensis (Hoyle 1885) [J].International Journal of Biological Macromolecules,2014,64:202-212.
[10] 顾青青,安叡,张艺竹,等.不同产地海螵蛸中核苷类成分测定[J].中成药,2015,37(5):1016-1021.
[11] SHUSHIZADEH M R,POUR E M,ZARE A,et al.Persian gulf β-chitin extraction from Sepia pharaonis sp.Cuttlebone and preparation of its derivatives[J].Bioactive Carbohydrates and Dietary Fibre,2015,6(2):133-142.

[12] LEE Y H,CHANG Y C,YAN H Y,et al.Early visual experience of background contrast affects the expression of NMDA-like glutamate receptors in the optic lobe of cuttlefish,Sepia pharaonis[J].Journal of Experimental Marine Biology and Ecology,2013,447:86-92.
[13] HOQUE M S,BENJAKUL S,PRODPRAN T.Effects of hydrogen peroxide and Fenton's reagent on the properties of film from cuttlefish (Sepia pharaonis) skin gelatin[J].Food Chemistry,2011,128(4):878-888.

[14] 陈道海,郑亚龙.虎斑乌贼(Sepia pharaonis)繁殖行为谱分析[J].海洋与湖沼,2013,44(4):931-936.
[15] 陈道海,王雁,梁汉青,等.虎斑乌贼(Sepia pharaonis)胚胎发育及孵化历期观察[J].海洋与湖沼,2012,43(2):394-400.
[16] 黄建盛,陈刚,张健东,等.野生虎斑乌贼(Sepia pharaonis)肌肉主要营养成分分析及评价[J].营养学报,2014,36(5):502-504.
[17] 高晓兰,蒋霞敏,乐可鑫,等.野生虎斑乌贼不同组织营养成分分析及评价[J].动物营养学报,2014,26(12):3858-3867.
[18] 蒋霞敏,彭瑞冰,唐锋,等.虎斑乌贼规模化人工繁育技术研究[C]//2013年全国海水养殖学术研讨会论文集.绍兴:中国水产学会,浙江省水产学会,2013:46-47.
[19] 赵云涛,李琨,黄燕,等.海螵蛸入药部分和废弃部分8种无机元素分析[J].微量元素与健康研究,2003,20(2):35-37.
[20] 杨振萍,边清泉.火焰原子吸收光谱法测定海螵蛸中15种微量元素含量[J].食品科学,2010,31(6):190-192.
[21] 李兰,吴启南.海螵蛸的化学成分研究[J].现代中药研究与实践,2009,23(2):52-54.
[22] 中华人民共和国卫生部,中国国家标准化管理委员会.GB/T 5009.3-2003 食品中水分的测定[S].北京:中国标准出版社,2004:25-29.
[23] 中华人民共和国卫生部.GB 5009.4-2010食品中灰分的测定[S].北京:中国标准出版社,2010:1-2.
[24] 中华人民共和国卫生部,中国国家标准化管理委员会.GB/T 5009.5-2003食品中蛋白质的测定[S].北京:中国标准出版社,2004:37-41.
[25] 中华人民共和国卫生部,中国国家标准化管理委员会.GB/T 5009.6-2003食品中脂肪的测定[S].北京:中国标准出版社,2004:45-46.
[26] 郭金龙,陈有君,孙国琴,等.苯酚-硫酸法测定杏鲍菇多糖方法的研究[J].食品科学,2008,29(12):555-558.
[27] 孙磊,乔善义,赵毅民.黑骨藤多糖含量测定方法研究[J].中国中药杂志,2009,34(10):1241-1244.
[28] 张惟杰.糖复合物生化研究技术[M].杭州:浙江大学出版社,1999:11-12.
[29] 中华人民共和国卫生部,中国国家标准化管理委员会.GB/T 5009.124-2003食品中氨基酸的测定[S].北京:中国标准出版社,2004:115-119.
[30] 姚林杰,叶元土,蔡春芳,等.饲料蛋白质脂肪比与不同生长阶段团头鲂全鱼蛋白质、脂肪含量及肌肉氨基酸、脂肪酸组成的关系[J].动物营养学报,2014,26(8):2184-2196.
[31] DAUPHIN Y.The organic matrix of coleoid cephalopod shells:molecular weights and isoelectric properties of the soluble matrix in relation to biomineralization processes[J].Marine Biology,1996,125(3):525-529.
[32] 肖述.乌贼海螵蛸形成机理研究[D].硕士学位论文.青岛:中国海洋大学,2003.
[33] 赵中杰,江佩芬,李昂.海螵蛸中碳酸钙、微量元素和氨基酸的测定[J].中国中药杂志,1990,15(1):41-43.
[34] KEITH J,STOCKWELL S,BALL D,et al.Comparative analysis of macromolecules in mollusc shells[J].Comparative Biochemistry and Physiology Part B:Comparative Biochemistry,1993,105(3/4):487-496.
[35] WEINERS S,HOOD L.Soluble protein of the organic matrix of mollusk shells:a potential template for shell formation[J].Science,1975,190(4218):987-989.

[36] KAPLAN D L.Mollusc shell structures:novel design strategies for synthetic materials[J].Current Opinion in Solid State and Materials Science,1998,3(3):232-236.

[37] MANN S.Molecular recognition in biomineralization[J].Nature,1988,332(10):119-124.
[38] 刘建军,陈卫红.中华猕猴桃微量元素测定分析[J].广东微量元素科学,2001,8(4):64-65.
[39] 夏静芬,钱国英,陈亮,等.珍珠粉和贝壳粉的化学成分和结构特征分析[J].化学研究与应用,2010,22(11):1467-1471.
[40] 孙会玲,陈庆国,刘梅,等.天然材料贻贝壳的应用研究[J].安徽农业科学,2014,42(13):4069-4071,4101.
[41] The Commission of the European Communities.(EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs[S].[S.l.]:Official Journal of the European Union,2006:L364/5-L364/24.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References