This experiment was conducted to study the effects of bile acid supplemental level on the growth performance, physical indices and body composition of juvenile Schizothorax prenanti. A total of 360 healthy juvenile Schizothorax prenanti with the average body weight of (12.74±0.14) g were randomly divided into four groups with three replicates of 30 fish per replicate. Each group was fed a experimental diet containing either 0 (control group), 75, 150 or 300 mg/kg bile acid for 70 days. The results showed as follows:with bile acid supplemental level raising, the weight gain rate (WGR), specific growth rate(SGR), protein efficiency ratio (PER) had a trend of going up first and then leveled off. Conversely, the feed conversation ratio (FCR) was firstly decreased and then to stabilization. The WGR, SGR and PER in bile acid supplemental groups were significantly higher than those in control group (P<0.05), while the FCR in bile acid supplemental groups was significantly lower than that in control group (P<0.05). The WGR and SGR were all the highest (226.63% and 1.69%/d, respectively) when the bile acid supplemental level was 150 mg/kg, and the PER was the highest (1.64%) when the bile acid supplemental level was 300 mg/kg, while the FCR was the lowest (1.56) when the bile acid supplemental level was 75 mg/kg. Based on square regression analysis, the suitable bile acid supplemental level was 186.83, 192.86, 166.67 and 183.33 mg/kg, respectively, when WGR, SGR, PER and FCR of juvenile Schizothorax prenanti were optimum. With bile acid supplemental level raising, the condition factor (CF) was initially increased and then leveled off, the viscerasomatic index (VSI) and hepaticsomatic index (HSI) were initially increased and then decreased. The CF in bile acid supplemental groups was significantly higher than that in control group (P<0.05), while the VSI and HSI in bile acid supplemental groups were significantly lower than those in control group (P<0.05). The crude protein content of whole body and muscle was initially increased and then to stabilization with bile acid supplemental level raising, while the crude lipid content of whole body, muscle and hepatopancreas was initially decreased and then leveled off. There were no significant differences in moisture and crude ash contents of whole body and muscle with bile acid supplemental level raising (P>0.05). Results of above show that bile acid supplementation can improve feed efficiency, promote growth, improve physical indices and reduce fat deposition in muscle and liver of juvenile Schizothorax prenanti. After comprehensive consideration, the suitable bile acid supplemental level in juvenile Schizothorax prenanti diet is 166.67 to 192.86 mg/kg.