Effects of Prolactin on Gene Expressions Involved in Milk Fat and Milk Protein Synthesis in Bovine Mammary Epithelial Cells

XING Yuanyuan; LI Dabiao; LI Honglei; YU Yongqiang; WANG Weiyun

doi:10.3969/j.issn.1006-267x.2016.08.015

2016 , Vol. 28 >Issue 8: 2439 - 2447

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.08.015

MOLECULAR NUTRITION

Effects of Prolactin on Gene Expressions Involved in Milk Fat and Milk Protein Synthesis in Bovine Mammary Epithelial Cells

XING Yuanyuan ,
LI Dabiao ,
LI Honglei ,
YU Yongqiang ,
WANG Weiyun

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College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2016-03-01

Online published: 2016-08-17

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Abstract

The aim of this study was to determine the effects of prolactin on gene expressions involved in milk fat and milk protein synthesis in bovine mammary epithelial cells (BMECs). mammary epithelial cells of Chinese Holstein cows were used, after purification culture, different concentrations[0 (control), 100, 300, 500 and 1 000 ng/mL] of prolactin were added in culture medium for a 24 h cultivation. Cell viability was detected by thiazoly blue tetrazolium bramide (MTT); triglyceride (TAG) content was measured by using TAG determination kit; gene expressions involved in milk fat and milk protein synthesis were measured by real-time quantitative PCR (RT-qPCR). The results showed as follows:1) 100 and 300 ng/mL prolactin significantly promoted the proliferation of BMECs compared with control and other experimental groups (P<0.05). 2) The content of TAG in BMECs and gene expressions of acetyl-CoA carboxylase (ACC), diacylgycerol acyltransferase (DGAT), fatty acid-binding protein 3 (FABP3) were significantly higher in 300 ng/mL prolactin group compared with those in control group (P<0.05), and gene expressions of stearoyl-coenzyme A desaturase (SCD) and peroxisome proliferator-activated receptor γ (PPARγ) tended to be higher. 3) Compared with control group, 100 and 300 ng/mL prolactin significantly upregulated gene expressions of mammalian target of rapamycin (mTOR) and prolactin receptor (PRLR) (P<0.05), and 300 ng/mL prolactin significantly upregulated αS1 casein (CSN1S) gene expression (P<0.05). In conclusion, 100 to 300 ng/mL prolactin is an optimal level considering its improvement effects on milk fat and milk protein synthesis.

Key words： bovine mammary epithelial cells; prolactin; cell viability; triglyceride; genes related in milk fat and milk protein synthesis

Cite this article

XING Yuanyuan , LI Dabiao , LI Honglei , YU Yongqiang , WANG Weiyun . Effects of Prolactin on Gene Expressions Involved in Milk Fat and Milk Protein Synthesis in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2016 , 28(8) : 2439 -2447 . DOI: 10.3969/j.issn.1006-267x.2016.08.015

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