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Effects of Stress and Dietary Energy Level on Fatty Synthesis in Liver of Broiler Chickens

  • CAI Yuanli ,
  • LIN Hai
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  • 1. College of Life Sciences, Qilu Normal University, Jinan 250013, China;
    2. Department of Animal Science, Shandong Agricultural University, Taian 271018, China

Received date: 2016-02-02

  Online published: 2016-08-17

Abstract

The objective of this study was to explore fatty acid synthase (FAS) activity, expressions of AMP-activated protein kinase (AMPK) and sterol-regulatory element binding proteins-1 (SREBP-1) mRNA in liver of broiler chickens treated by glucocorticoid or dietary energy level in order to explain why stress can raise the hepatic triglyceride and look for ways to relieve stress. There were four trials in this study. In the first and the second trials, broiler chickens fed high and low different energy level of diets were challenged with corticosterone from 3 to 9 days of age and 28 to 34 days of age, respectively. At the end of the two trials, livers were removed for measuring FAS activity. In the third trial, 108 male Arbor Acres chickens with 7 days of age were divided into 3 groups injected with dexamethasone (stress group), saline (control group) and saline (a pair-fed group, with the same feed intake compared to stress group) for 7 d, respectively. At the 14 days of age, the liver was removed to determine the triglyceride content and expressions of AMPK and SREBP-1 mRNA. In the last trial, chickens aged from 3 to 9 days were treated with corticosterone and drinking water supplemented with glucose, and FAS activity was measured in the end. The results showed as follows:1) FAS activity was significantly increased by corticosterone treatment in chickens of 3 to 9 days old (P<0.05), and had the increasing trend in chickens of 28 to 34 days old (P=0.051 2). 2) Compared with the pair-fed group, dexamethasone administration resulted in enhanced triglyceride content in liver (P<0.05), and the AMPK mRNA expression level in liver was significantly up-regulated (P<0.05). The SREBP-1 mRNA expression level in liver of chickens treated with dexamethasone were significantly higher than those of control and pair-fed groups (P<0.05). 3) Glucose supplementation could significantly decrease the FAS activity in liver of chickens during stress (P<0.05). In conclusion, corticosterone treatment can increase the FAS activity in liver of broiler chickens, dexamethasone administration can increase SREBP-1 mRNA expression level in liver and activate AMPK, and glucose can relieve the harm of stress.

Cite this article

CAI Yuanli , LIN Hai . Effects of Stress and Dietary Energy Level on Fatty Synthesis in Liver of Broiler Chickens[J]. Chinese Journal of Animal Nutrition, 2016 , 28(8) : 2512 -2520 . DOI: 10.3969/j.issn.1006-267x.2016.08.023

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